Effects Of AGEs On Invasion And Migration Of Colon Cancer HCT116 Cells In Hypoxic Microenvironment

Objective:To investigate the effects of AGEs on proliferation,invasion,migration and EMT of colon cancer HCT116 cells in hypoxic microenvironment.Methods:The best AGEs concentration(200μg/mL),and the best action time(48hours),promoting the proliferation of HCT116 cells were screened by CCK-8 assays.Then HCT116 cells were divided into blank control group,AGEs(200μg/mL)group,hypoxia group and hypoxia+AGEs(200μg/mL)group.The cell cycles were detected by flow cytometry,the invasive and migratory abilities of HCT116 cells in vitro were detected by Transwell chamber invasion and migration assays.The expression of proteins HIF-1α,RAGE,E-cadherin,N-cadherin,Vimentin and MMP2 in four groups were determined by Western blotting.Results:Compared with the blank control group,hypoxic microenvironmentpromoted the proliferation of HCT116 cells,but AGEs had little effect on the proliferation of HCT116 cells.The abilities of cell invasion and migration increased gradually in the other three groups in vitro invasion and migration experiments compared with the blank control group.Western blotting showed that the expression level of HIF-1α was low in normoxic environment,but increased significantly in hypoxic condition.At the same time,the expression of HIF-1α increased further after adding AGEs in hypoxic condition.The expression of RAGE increased in both normal and hypoxic environments after adding AGEs,especially in hypoxic environment.Compared with the blank control group,the expression of MMP2,N-cadherin and Vimentin increased gradually in the other three groups,while the expression of E-cadherin decreased gradually.Conclusion:AGEs further enhances the invasive and migratory abilities of HCT116 cells under hypoxic environment,but has little effect on the proliferation of HCT116 cells.