The Effects Of RNF10 On The Function Of Vascular Smooth Muscle Cells Induced Exposed To High Glucose

OBJECTIVES:To investigate the protective effects of RNF10 on vascular smooth muscle cells（VSMCs）exposed to high glucose.METHODS:Vascular smooth muscle cells（VSMCs）were induced by high glucose（4.5g/L）to proliferate excessively and deposit extracellular matrix in vitro.According to the culture medium and adenovirus transfection,the cell experiment was divided into four groups:Control group;High glucose group（HG group）;High glucose+Adenovirus Green fluorescent protein group（HG+Ad-GFP group）;High glucose+overexpression RNF10 group（HG+Ad-RNF10 group）.Qualitative analysis of VSMCs adenovirus transfection rate was observedunder fluorescence microscope;Both CCK-8 and EdU assays were used to detect VSMCs proliferative rate;Flow cytometry verified cell cycle distribution of VSMCs;Western blot analyzed the expressions of RNF10、CyclinD1and CDK4 protein in VSMCs;qPCR analyzed the expressions of MMP-2and TIMP-2 mRNA in VSMCs.Results:（1）RNF10 significantly inhibited the proliferation of VSMCs exposed to high glucose（P<0.05）;（2）RNF10 significantly inhibited the transition from G₀-G₁ to S phase of VSMCs exposed to high glucose（P<0.05）;（3）RNF10 significantly inhibited the expressions of Cyclin D1 and CDK 4 in VSMCs exposed to high glucose（P<0.05）;（4）RNF10 significantly inhibited the transcription levels of MMP-2 and TIMP-2（both P<0.05）,however the inhibition of TIMP-2 was more obvious.Eventually RNF10 significantly inhibited of MMP-2/TIMP-2（P<0.05）.CONCLUSION:RNF10 can significantly improve the function of VSMCs exposed to high glucose,which may be related to the inhibition of VSMCs proliferation and extracellular matrix accumulation.