Isolation Of Antibacterial Components Of Two Arthropod Insects And Small Molecular Probe Of Natural Products Chemicobiology

It is a rare or unusual resources for insect living in polluted enviroment with the novel antibacterial structures.As a rising multidisciplinary field,natural product resource chemistry and the natural products are being paid much more developing.After optimizing correlative methodology and technology,we have continuously studied and investigated the antimicrobial components of selected arthropod insects,adopting the basic theory and method of natural product research,and combining the isolation skills and structural analysis means.In this paper,studies were carried out on the extraction,isolation,identification and structure identification of arthropod insects Aspongopus chinensis and Huechys sanguinea secondary metabolites,based on our early established biological two-stage extraction method studied on the insect chemistry studies.On the other hand,according to the research of our group over the years about the insets antibacterial,this paper explored the further develop study of small molecular probe construction based on the novel structure of the antibacterial activity of small molecules.The results showed that eight compounds were obtained from antibacterial activity of methanol and 70% methanol in two arthropod insects.Among them,there are two new N-propionyldopamine derivatives,two new oxazole compounds with the same origin and one nitrogen glycoside compound for total five new compounds along with three known compounds.Of which new compounds were respectively named as: 8-N-propylamidyl,14-N-ethylamideyl –dopaminedimers(Compound 1),7,8-dihydron-(N-propylamidyl),15-pyrazine-N-ethylamideyl-dopaminedimers(Compound 2),1-[1'-o-aldehyde phenyl]-3-methoxyl-4-methylaminoyl-oxazole(Compounds 3),1-[1'-o-aldehyde phenyl]-3-actyl-(6-methoxyl)ester,4-methylaminoyloxazole(Compounds 6)and Dihydron-pyridinyl-?-glucosyl-[1-1']-N-glycoside(Compound 8).And three known compounds are the 5-methylhexahydropyrroleone(compound 7),Allantoin(compound 4),and Malonyl glyceridester(compound 5).Their biological activities were investigated on the antibacterial evaluation and liturure research,revealed the compound 1 and 2 with antitumor activity,compound 3 and 6 with antifungal activity and compound 4,5 and 7 used widely as skin conditioner in cosmetic industry.Then,by studying on the biological functions of the two kinds of insects,the medicinal substance basis of Aspongopus chinensis was clearly elucidated,and the biological activity of Aspongopus chinensis was mainly anticancer and antiinflammatory Moreover,this work also extended to a newly cross discipline between chemistry and biology for the insect-derived chemicobiological information in preliminary our study on antimicrobial compounds.This paper mainly focuses on the basic theoretical research of natural product in chemical biology and natural product small molecule probe construction.The results were also concerned with the characterization of active molecular biological titers of natural products and the calculation of the electron structure of antimicrobial compounds by density functional theory calculation.In this work,it displayed that arthropod insects have the great potential in the antibacterial drugs discovery through the continuous separation and purification of the antibacterial components and the antibiotic-like substances from arthropod insects living in polluted enviroment.Meanwhile,an extended research has been carried out in the application of novel structures from insect.This is the first reported of the characterization of bioavailability of active molecules and the exploration of natural products small molecular probes construction,as well as the basis of medicinal substances of Aspongopus chinensis clarified in this paper,since that are likely to prove one of the highlights of this study.