Effects Of High-fat Diet On Blood Lipids And Semen Quality In Men And Male Rats

Objective:The blood lipid and semen quality are detected to explore the differences of blood lipid and semen quality between the high-fat diet mice and the standard diet mice through the establishment of high-fat diet model and standard diet model.The differences of blood lipid level,semen quality and serum sex hormones between high-fat diet men and non-high-fat diet men are further explored through filling in dietetic questionnaires,detecting blood lipid level,semen quality and serum sex hormones.The relationship between hyperlipidemia and semen quality is further explored.Providing suggestions about diet structure and blood lipids regulation for infertile patients caused by high-fat diet.Methods one:a study of mouse model?1?Male mice at the age of 6-8 weeks are divided into HFD and SD groups.HFD means that mice are fed by food which containing 60%fat while SD means that mice are fed by food which containing 10%fat.After 8 weeks or 23 weeks,the mice are measured the last weight,the coefficient of testis and epididymis.Testicular and epididymis are observed under microscope after HE staining,then the differences of reproductive organs,tissues and cytology between different groups are discussed.?2?Automatic biochemical analyzer are used to measure the differences of serumTG,TC,HDL-Cand LDL-C.The differences of blood lipid are discussed between different groups.?3?Microscope is used to observe the sperm vitality and count.Using the sperm?cell?morphological rapid dye staining to stain sperm and observe the abnormal sperm.The differences of semen quality in different diet groups are discussed.Methods two:a clinical study?1?108 men who are conformed to inclusion and exclusion criteria and divided into HFD and NHFD groups through dietary questionnaire are investigated.Automatic biochemical analyzer are used to measure their serum lipid(TG,TC,HDL,LDL,Apo-AI,Apo-B and Lp-a.Measuring the serum sex hormones?FSH,LH,T,PRL,E₂?to discuss the differences of blood lipid and serum sex hormones between different diet groups.?2?Computer aided semen analysis system are used to measure the SC,the count of normal morphology sperm,PR%,NP%,IM%,the total sperm count and the forward movement sperm count.Using the sperm?cell?morphological rapid dye staining to stain sperm and then counting the rate of sperm deformity.Discussing the different sperm qualities between different groups.Results:?1?HFD for 8 weeks group compared to SD for 8 weeks group,TC,TG,LDL-C and HDL-C increased,which have statistical differences?p<0.05?.?2?HFD for 8 or 23 weeks group compared to SD groups have increased sperm deformity rate,decreased total sperm count,sperm vitality,coefficient of testis and epididymis,the epithelial thickness and diameter of testicular seminiferous tubule,the number of secondary spermatocyte added sperm cells and sperm in tube count.HFD for 23 weeks group compared to SD for 23 weeks group also has decreased epithelial thickness and diameter of epididymis and the number of spermatogonium,which all have statistical differences?p<0.05?.Hyperlipidemia for 8 weeks group compared to Non-hyperlipidemia for 8 weeks group,the rate of sperm abnormality are increased,the sperm count and vitality are decreased,which all have statistical differences?p<0.05?.HFD group compared to NHFD group,the fat in the testicular tissue is increased,spermatogenic cells in the seminiferous tubule is decreased and arrange disorderly,some spermatogenic cells are apoptotic,necrotic and shedding.?3?HFD for 23 weeks group compared to HFD for 8 weeks group,total sperm count,sperm vitality and sperm deformity rate all don't have statistical difference?p>0.05?.The coefficient of epididymis,the diameter of testicular seminiferous tubule,the epithelial thickness of epididymis,the number of spermatogonium,the number of secondary spermatocyte added sperm cell and sperm count in tube are deceased,which all have statistical differences?p<0.05?.?4?People who belong to HFD group compared to NHFD group have higher TG,TC,LDL–C and lower HDL-C,ApoAI,Lp-a with statistical differences?p<0.05?.The SC,the percentage of normal sperm,PR%,the sperm count and the forward motile sperm count are increased with statistical differences?p<0.05?.IM%,LH and T are decreased with statistical differences?p<0.05?.?5?People who have hyperlipidemia compared to non-hyperlipidemia have decreased the SC,the percentage of normal sperm,PR%,the sperm count and the forward motile sperm count with statistical significances?p<0.05?.IM%is increased with statistical difference?p<0.05?.Conclusion:?1?HFD results in abnormal blood lipid for mice,which shows the characteristics of hyperlipidemia.HFD results in lower sperm count,lower sperm viability and higher sperm abnormalities.HFD cause the morphological changes of the Spermatogenic cell,tissue and organs,which affects the sperm production.?2?The semen quality of hyperlipidemia mice are decreased.?3?HFD results in abnormal blood lipid for people,which show the characteristics of hyperlipidemia.HFD men compared to NHFD men have lower sperm quality,LH and T.?4?Hyperlipidemia men has lower sperm quality.