The Experimental Study Of Staphylocaoocal Aureus Adaptation To Human PJI Fluid And Antimicrobial Characteristics Of Berberine Against S.aureus In PJI

OBJECTIVE: Prosthetic joint infection(PJI)is a disastrous complication of arthroplasty surgeries.Staphylococcal aureus is the leading causative pathogen and it's chararcterized by its strong virulences,biofilm formation ability and adaptation capacity.These features,as well as the high frequency of MRSA in clinic,make S.aureus induced PJI difficult to tackle.Thus,it's urgent to find a proper S.aureus strain as a tool to study the pathogenesis of PJI and a new antibacterial agent to achieve effective prophylaxis and treatment of S.aureus related PJI.METHODS: First,we isolated a PJI related S.aureus strain and determined its MLST type as ST1792,after which we did whole genome sequencing of ST1792.Then,we collected synovial fluid,plasmin and drainage fluid and performed biofilm assay test and transcriptomics analysis of ST1792 in these three human fluid.Beside,we tested the antimicrobial property of berberine against eighteen S.aureus isolated from PJI patient and conducted transcriptomics analysis of ST1792 in the presence of berberine.RESULTS:Whole genome sequencing revealed that ST1792 was a strain with a integral S.aureus genome.Bioflim assay showed that S.aureus formed greater biofilm in synovial fluid and plasma than drainage fliud compared to control group.Transcriptomics analysis indicated the sample of synovial fluid and plasma was close,while drainage fliud was quite different.Berberine exerted effective antibactrial property against eighteen PJI associated S.aureus strains with relation to their MLST types.But in some strains,low consentrations of berberine surprisingly enhanced biofilm formation.Transcriptomics analysis of ST1792 in berberine revealed berberine caused damage to bacterial DNA,disrupted cell division and energy metabolism process of S.aureus.CONCLUSIONS:ST1792 shows considerable adaptation in human PJI fluid and thus is an appropriate tool strain for PJI pathegenesis.Berberine has effective antibactrial property against eighteen PJI associated S.aureus possibly by damaging bacterial DNA,disrupting cell division and energy metabolism process of S.aureus.