Function And Molecular Mechanism Of The RNA Binding Protein ASS1 And CSRP1 In Lung Cancer

Lung cancer is the leading cause of cancer-related deaths among males worldwide.Lung cancer occurred most frequently and became the most common cause of cancer death in China,with the very low 5-year survival rate.However,the complex molecular and cellular mechanism involved in lung cancer metastasis are not fully elucidated.Therefore,it is urgent to explore the mechanism of lung cancer metastasis.Recent evidence has shown that RNA-binding proteins(RBPs)are involved in carcinogenesis and tumor progression.In this study,we aim to explore the effects of ASS1 and CSRP1--RNA-binding protiens--on proliferation,migration and invasion ability of human lung adenocarcinoma cell lines,and to explore its underlying mechanism.We constructed stably expressing ASS1 or CSRP1 lung cancer cells.Consequently,we detected both ASS1 and CSRP1 expression level by q RT-PCR as well as Western Blot assays.In addition,by performing CCK-8、Transwell assays and Wound healing assays,we found that ASS1 over-expression inhibited the migration and invasion abilities of lung cancer cells while overexpression of CSRP1 demonstrated the opposite phenomenon.Besides,CSRP1 facilitated the proliferation of lung cancer cells while ASS1 does not.As to mechanism study,we discovered that ASS1 may regulate the MAPK signaling pathway and CSRP1 may regulate the p-FAK signaling pathway.Furthermore,we observed that ASS1 interacted with CPS1 and regulated its expression in lung cancer cells.In summary,we reveal the biological function and underlying mechanisms of ASS1 and CSRP1 in lung cancer migration and proliferation,which may serve as novel therapeutic targets for lung cancer patients.