Rapid Detection Of Leukemia Based On Electrochemical Sensing

Leukemia is one of the diseases that are difficult to treat at present.Its early rapid detection is always the focus of research in biology,medicine and other related fields.Electrochemical sensing technology has been widely used in biomedicine,environmental monitoring,food and other fields because of its advantages of good selectivity,high sensitivity,rapid analysis and low cost.Nanomaterials have good optical,electrical,magnetic,biocompatibility and catalytic properties and they are similar to some biomolecules in sizes.It has become a hot topic for researchers to combine nanomaterials with electrochemistry for the building of nanoelectrochemical biosensor and further application in biomedical testing and research.Screen-printed carbon electrodes(SPCEs)are disposable electrodes.They have many advantages,such as low price,simplicity,fast preparation,mass production,without preprocessing and easy to realize commercialization.It can not only make the electrochemical biosensor easier,but also avoid cross contamination on the surface of the electrodes.They can also increase the possibility of instant detection.In view of these described above,the application of gold nanoparticles modified boron-doped nanocrystalline diamond microelectrode arrays(Au NPs-BNCD-MEAs)and SPCEs in the detection and recognition of different types of white blood cells was investigated and studied in this paper.In this paper,drug sensitive leukemia K562 cells,drug resistant leukemia resistant leukemia KA cells,healthy human leukocytes and leukemia cells were used as the research object.Au NPs-BNCD-MEAs and SPCEs were used to detect them by electrochemical methods.The results show that different types of white blood cells can be distinguished rapidly by using their significant differences in electrochemical properties,and their sensitive detection is achieved.This electrochemical detection technique is of great significance and potential application in the early diagnosis and monitoring of leukemia in clinical practice.