Effects Of Alcohol Treatment On Placental Development Of Preimplantation Embryosin Mouse And Repair Effects Of Betaine

A large number of studies have shown that drinking alcohol during pregnancy can lead to an increased risk of developing abnormal embryos.In our previous studies,the effects of alcohol on the development of embryos at various stages have been demonstrated.The placenta is responsible for the exchange of oxygen and nutrients in the development of mammals and for the production of various factors and hormones necessary to maintain a normal pregnancy.However,the long-term effects of alcohol exposure of early embryos on placental development have long been unclear.To further elucidate the role of placenta in alcohol-induced embryonic dysplasia,this study used embryonic in vitro culture and transplantation techniques to study the placental status of fetuses from pre-implantation alcohol-treated embryos to 13.5 days.In the study,the abnormal developmental phenotype and potential mechanism of placenta were analyzed by embryology,proteomics,immunohistochemistry and various other methods,and the possibility of betaine repairing placental damage was analyzed.The research results are as follows:1.Compared with the normal group,the pre-implantation embryos treated with alcohol can develop the placenta,but the placenta formation rate is reduced and the weight of the formed placenta is also significantly lower.The proportions of various structures in the placenta are significantly changed: the proportion of the junctional zone in the entire placenta was reduced,and the proportion of the labyrinth was expanded.2.Alcohol treatment of early embryo affects the function of placenta after implantation.Proteomic analysis showed that 510 proteins were significantly different expressed in the alcohol group,especially the proteins related to the modification and metabolism of sugars such as glycoproteins and polysaccharides.3.Alcohol treatment of preimplantation embryos disrupted the normal formation of blastocyst lineage,and the expression balance of lineage markers Cdx2 and Oct4 changed,resulting in abnormal developmental fate after implantation.4.Compared with the embryos treated with alcohol,the balance of Cdx2 and Oct4 expression in the abnormal treatment of embryos treated with betaine-treated alcohol was restored,and the proportion of placental labyrinth decreased after implantation,and the proportion of the junction area expanded.In summary,the results of this study indicate that the long-term effects of alcohol on embryonic development are characterized by transient embryonic early exposure to 1.0% alcohol,which still has significant damage effects on long-term embryo development,and the impact on placental development is an important part.