Pharmacologic Dose Of Vitamin C Along With IL-24-hBMSCs Targeting To Inhibit The Growth Of Colon Cancer Cell Line HCT116

Objective:The ultimate goal of cancer research is to explore sensitive targeted therapies that effectively kill mutant tumor cells without affecting normal cells.Gene therapy has become a research hotspot in tumor therapy.Studies have shown that stem cells have a tendency to tumor,and ascorbic acid(Vc),as a potential ideal anticancer drug,has not yet been elucidated in combination with IL-24.This study aims to explore the targeted anti-colon cancer drugs,use human bone marrow mesenchymal stem cells to build a cell model with stable expression of IL-24 protein,and jointly act on colon cancer with Vc,to study the combined anti-tumor effect and related mechanism.Methods:Human bone marrow mesenchymal stem cells(hBMSCs)were cultured by whole bone marrow adherent method.IL-24-hBMSCs model was constructed by lentivirus transfection.The expression of IL-24 in hBMSCs was detected by RT-PCR and Werstern blot.Cell toxicity was detected by CCK8 assay.Cell apoptosis was detected by flow cytometry.In vivo,the nude mouse model of colorectal cancer was divided into 5 groups:Vc group,IL24-hBMSCs group,Vc+IL24-hBMSCs group,DesRed-hBMSCs group,saline group.Vitamin C is given by intraperitoneal injection(100 mg/kg),while cells are injected by tail vein(10~7 cells),after 16 days of treatment.The size of the tumors was observed,and the histopathological changes of the tumors were observed by hematoxylin-eosin(HE)staining.The expression of apoptotic protein Bax/caspase-3 were detected by Western blot.Results:Flow cytometry confirmed that hBMSCs were successfully obtained by whole bone marrow adherent method.The IL-24 gene and protein were confirmed by RT-PCR and Western Blotting.In cell experiment,Vc+IL-24 group showed increased apoptosis,and the difference was statistically significant.In vivo,Vc+IL-24-hBMSCs group showed significantly smaller tumor size than the other groups,and HE staining showed that the tumor in this group had the most necrotic areas and increased apoptotic protein expression.Conclusion:Pharmacologic dose of Vc can enhance the anti-colon cancer effect of IL-24-hBMSCs.