Research On The Effect Of FBXL5 On TNBC EMT Progress And The Mechanism Explore

Objective:(1)Explore FBXL5 expression in triple-negative breast cancer tissues and different types of breast cancer cells compared with adjacent normal breast tissues or normal breast cells;(2)Study the effect of FBXL5 on EMT progress in vitro and explore the proper mechanism.METHODS:(1)1.Using RT-PCR and Western Blot methods to explore the expression of FBXL5 in TNBC tissues and adjacent tissues;(2)Use Western Blot to explore FBXL5 expression in MCF-7,BT549,MDA-MB-231,MDA-MB-453 cancer cells and normal breast cells;(2)LV-FBXL5 and negative-control(LV-NC)were synthesized and MDA-MB-231 cells were infected with LV-FBXL5 or LV-NC.The effection of infection was tested by Western Blot and RT-PCR;(3)Transwell and scratch test were tested to verify the FBXL5's effection on the invasion and metastasis ability of MDA-MB-231 cells;(3)Western Blot was used to test the changes of EMT-related proteins after infection of LV-FBXL5 or LV-NC;(4)The p-ERK and p-p38 protein levels were tested by Western Blot after infection of LV-FBXL5 or LV-NC;RESULTS:(1)RT-PCR and Western blot's results showed that the mRNA and protein level of FBXL5 in TNBC tissues was lower compared with its parental;(2)The protein levels of FBXL5 in MCF-7?BT549?MDA-MB-231and MDA-MB-453 breast cancer cells all decreased and most obviously in MDA-MB-231 cells(p<0.05);(3)The FBXL5-overexpression could be achieved by infection with LV-FBXL5 compared with LV-NC;(4)Overexpression of FBXL5 could inhibit the ability of invasion and metastasis of MDA-MB-231 cells;(5)Overexpression of FBXL5 could inhibit the progress of EMT by increase the expression of E-cadherin and decrease the protein level of Vimentin;(6)The p-ERK and p-p38 protein levels were decreased by overexpression of FBXL5 and thus inhibit the activation of the ERK and p38pathways?Conclusion:FBXL5 was decreased in TNBC tissues and breast cancer cells.Overexpression of FBXL5 by infection with LV-FBXL5 could inhibit the progress of EMT in vitro,the mechanism was properly related with inhibiting the activation of ERK and p38pathways.