The Effects Of HGSTP1 On Radiation-induced Lung Cell Injury

ObjectiveTo study the effect of human glutathione S-transferase PI?GSTP1?protein on radiation-induced lung cell injury.Mimicking the decline of GSTP1 activity,in order to find the basic experimental evidence for the phenomena that GSTP1 is related to the incidence of radiation induced lung injury.Methods1?Using GSTP1 siRNA and Overexpression plasmid to modulate the expression of GSTP1.2?Protein expression is determined by RT-qPCR and western blot.3?MTT assay was performed to measure the proliferation of BEAS-2B,and the growth curve was drawn based on the results.4?Radiosensitivity of A549 was tested by clone formation assay.5?DCFH-DA probe was used for incubation.Flow cytometry was conducted to detect the median fluorescence intensity?MFI?and cellular apoptosis.Annexin-v/PI staining was utilized for incubation.6?MDA was measured by colorimetric assay.Results1?GSTP1 protein in lung will fall to a low level after ?-ray radiating that of a mice.2?Transfection of GSTP1 siRNA will reduce the expression and activity of GSTP1.3?Inhibitory effect of ⁶⁰Co?-ray irradiation on cell proliferation was enhanced by interfering with GSTP1 protein?in the fifih day,OD values of negative control and experimental groups are 1.035±0.041 VS 0.626±0.054/0.366±0.032,P<0.05?.4?After interfering with GSTPI protein,cell radiosensitivity was increased.D0,a Parameter of radiosensitivity,is smaller in experimental groups than that in control group.5?GSTP1 proteins affect the oxidative stress state of cells,and interfering with GSTP1 proteins will aggravate oxidative damage.6?Intervening GSTP1 will promote apoptosis induced by radiation?the apoptosis percentage of negative control and experimental groups are 12.33±1.16%VS 17.38±1.65%/22.88±1.20%?.7?Intervening GSTP1 will facilitate the progression of Epithelial-Mesenchymal Transition.ConclusionsThe effects of radiation on pulmonaiy epithelial cells were simulated by regulating the expression of GSTP1.The decrease of GSTP1 protein expression and activity will aggravate the oxidative damage of cells induced by radiation,promote the apoptosis induced by radiation,increase the radiosensitivity of cells and promote the EMT process.GSTP1 may alleviate radiation-induced lung injury by alleviating oxidative stress in cells...