The Effect Of Arctium On Breast Cancer Cell MCF-7

Objective: To investigate the mechanism of arctiin on the growth of breast cancer cells.Methods: Breast cancer cells and arctiin were used for cell and animal experiments.1.The MCF-7 cells of breast cancer were cultured,and the control group and the medication group were set up.MCF-7 breast cancer cells were cultured in different concentrations of arctiin in the experimental group.In the control group,only the same amount of nutrient solution was added.After culture,the relative viability(absorbance value)of breast cancer cells was detected by MTT assay.The inhibition rate of cell proliferation was calculated.The experimental data of each group were compared and analyzed.To reveal the effect of arctiin on the growth of breast cancer cells.2.Twenty female nude mice of similar size and weight were inoculated with breast cancer cell MCF-7 suspension 0.2ml(about 1 X107/L)on the back of nude mice for 10 days.They were randomly divided into control group,low-dose arctigenin group,middle-dose arctigenin group and high-dose arctigenin group.Each group was numbered 1-4 in turn.Five mice in each group were given intraperitoneal injection once a day and the same dose was given respectively.After 28 days of continuous injection,the nude mice were killed by pulling off the cervical spine 7 days later.The tumors in nude mice were cut off and weighed,and the anti-tumor rate of transplanted tumor cells was calculated.The expression of vascular endothelial growth factor(VEGF)was detected by immunohistochemical SP method and stained with aminobenzene(DAB).Results: Cell experiments showed that the 24-hour half inhibitory concentration of MCF-7 was about 1.42 mg/L.Nude mice experiments showed that the weight of breast cancer in 1-5 groups was(1.56+0.41g),(0.87+0.25g),(0.78+0.35g),(0.64+0.37g),respectively.T-test and bilateral test were used among the groups.The detection standard was 0.05,P < 0.05.There was significant difference in tumor weight among the groups,with statistical significance.IPP software was used to measure the integrated optical density(IOD)of breast cancer tumor VEGF in 1-5 groups of mice,which were(0.75+0.05),(0.58 +0.02),(0.51 +0.03),(0.42 +0.02).T-test was also used,P < 0.05.The IOD values of each group were significantly different.Conlusions: One of the anti-breast cancer mechanisms of arctiin is to inhibit the expression of vascular endothelial growth factor in breast cancer cells.