Analysis Of Differential Expression And Clinicopathological Significance Of CPONE1,KCTD14 And FUNDC2 Between Luminal Subtype Of Breast Cancer And Triple Negative Breast Cancer

Objective To investigate the differential expression and clinical pathological significance of CPNE1,KCTD14 and FUNDC2 proteins between luminal subtype of breast cancer and triple negative breast cancer.Then explore the possibility that they can be used as therapeutic targets for TNBC,providing more ideas for the treatment of TNBC.Methods Immunohistochemistry was used to detect the expression of CPNE1,KCTD14 and FUNDC2 in 82 cases of luminal subtype breast cancer,58 cases of triple negative breast cancer and 33 cases of non-tumor benign lesions,and to analyze their association with related clinicopathological features.Results1.CPNE1,KCTD14 and FUNDC2 were mainly expressed in human breast cancer cytoplasm.2.The expression of CPNE1 protein in breast cancer and non-tumor benign lesions was statistically significant(P<0.05),as well as in luminal subtype and TNBC(P<0.05).Its expression level was correlated with primary tumor size of breast cancer(P<0.05),and there was no significant correlation with the clinicopathological features such as age,lymph node metastasis and Grade classification(P > 0.05).There was a positive correlation between the expression of CPNE1 and KCTD14 in breast cancer(P < 0.05,r=0.336)and a positive correlation with the expression of FUNDC2 in breast cancer(P < 0.05,r=0.335).There was a negative correlation between the expression of CPNE1 and ER,PR and HER2(P<0.05,r:-0.548,-0.519,-0.37,respectively).3.There was no significant difference in the expression rate of KCTD14 between breast cancer and non-tumor benign lesions(P>0.05),but its expression in luminal subtype and triple negative breast cancer was statistically significant(P < 0.05).There was no significant correlation between the expression and the clinicopathological features such as age,primary tumor size,lymph node metastasis,and Grade classification(P>0.05).KCTD14 was positively correlated with FUNDC2 expression(P<0.05,r=0.245)in breast cancer.But there was no correlation between the expression of KCTD14 and ER,PR and HER2(P>0.05).4.The expression of FUNDC2 protein in breast cancer and non-tumor benign lesions was statistically significant(P<0.05),as well as in luminal subtype and TNBC(P< 0.05).FUNDC2 protein expression level was correlated with age(P<0.05),and there was no significant correlation with the clinicopathological features such as primary tumor size of breast cancer,lymph node metastasis and Grade classification(P>0.05).There was a negative correlation between the expression of FUNDC2 and ER,PR and HER2.(P<0.05,r:-0.200,-0.240,-0.332,respectively).Conclusion1.In breast cancer tissues,the expression levels of CPNE1 and FUNDC2 were higher than those in non-tumor benign lesions.The expression levels of three proteins in triple-negative breast cancer tissues were higher than those in luminal subtypes.The expression level of CPNE1 protein was correlated with the primary tumor size of breast cancer,the expression level of FUNDC2 was related to the age of the patients.2.The expressions of CPNE1,KCTD14 and FUNDC2 were positively correlated in breast cancer.There was a negative correlation between CPNE1 and ER,PR and HER2,as well as FUNDC2.