Effect Of ECEL1 Knockdown On Proliferation Of Hepatocellular Carcinoma Cells

Objective: To study the effect of ECEL1 gene on the proliferation of hepatocellular carcinoma cells,and provide a new target for the prevention and treatment of hepatocellular carcinoma.Methods:(1)ECEL1 gene RNAi lentivirusin infect human hepatocellular carcinoma cells,and set the control group at the same time,then fluorescence observation showed infection rate.(2)qPCR detect the mRNA expression of ECEL1 gene knockdown in hepatocellular carcinoma cells.(3)Western Blot endogenous verify the protein expression of ECEL1 gene in HCC cells.(4)Clonal formation detects cell proliferation,Celigo detects and records the number of cells at each time point for 5 consecutive days,so as to analyze the growth and proliferation of the cells.MTT detect its proliferation in this same way.Results:(1)ECEL1 gene RNAi lentivirusin infect human hepatocellular carcinoma cells(BEL-7404 ? Huh-7),Fluorescence observation under the microscope after 72 hours showed that the efficiency of cell infection was over 80% and the cells were in normal state.(3)qPCR detected The mRNA expression level of ECEL1 gene in BEL-7404 and Huh-7 cells were significantly inhibited(p<0.05),and the knockdown efficiency was 70.5% and 60.7% respectively.(4)The results of Western Blot endogenous validation showed that the protein expression level of ECEL1 gene in the experimental group was significantly down-regulated compared with control group(p<0.05).(5)The detection results of Clonal formation?Celigo and MTT show that compared with the control group,the experimental group has an obvious inhibition against the proliferation of BEL-7404 and Huh-7 cells.Conclusion:Knockdown the ECEL1 gene can inhibit the proliferation of human hepatocellular carcinoma cells.