| Objective By observing the different concentrations of rheumatoid one on the morphology,Cytokine and the degree of synovial tissue pathological damage of adjuvant arthritis rats(AA),to explore the effect and mechanism of rheumatoid one on AA rats,try to provide a theoretical basis for clinical treatment.Method Reproduction of rheumatoid arthritis rat model by using complete Freund's adjuvant,randomly divided into normal group,model group,tripterygium wilfordii tablets group,rheumatoid one high dose group,rheumatoid one medium dose group,rheumatoid one low dose group with continuous administration for 4 weeks,to observe the change of weight,toe swelling,arthritis index,,TNF-?,IL-6,DKK1,?-catenin and pathological of synovial tissue.Results 1.The weight:Compared with the normal group,the weight gain of each group was slower(P<0.05);compared with the model group,the tripterygium wilfordii tablets group,the high dose group,and the medium dose group were higher after 2 weeks of administration(P<0.05);there was no significant difference between the high dose group and the medium dose group after 3 weeks(P>0.05).2.Arthritis index:Compared with the normal group,the arthritis index of each group was significantly different(P<0.01).Compared with the model group,the tripterygium wilfordii tablets group,high dose group and medium dose group was significantly difference after 4 weeks of administration(P<0.05);there was no significant difference between the high dose group and the tripterygium wilfordii tablets group(P>0.05).3.Toe swelling degree:Compared with the normal group,The degree of swelling was higher(P<0.01).Compared with the model group,the rats in the tripterygium wilfordii tablets group,high dose group and medium dose group decreased after 4 weeks of administration(P<0.05),compared with tripterygium wilfordii tablets group,the difference between high dose group and medium dose group was statistically significant(P<0.05);4.TNF-?:Compared with normal group,the model group was higher(P<0.01).Compared with group model,the tripterygium wilfordii tablets group,high dose group and medium dose group was lower(P<0.01),serum TNF-? was lower in low dose group(P<0.05),Compared with the tripterygium wilfordii tablets group,there was no significant difference between the high dose group and the medium dose group(P>0.05),the low dose group was statistically significant(P<0.05).IL-6:Compared with the nomal group,the model group was higher(P<0.01);the tripterygium wilfordii tablets group,the high dose group,and the medium dose group were lower(P<0.05),compared with the model group,there was no significant difference in the low dose group(P>0.05);compared with tripterygium wilfordii tablets group,there was no significant difference between high dose group and medium dose group(P>0.05),and low dose group was higher(P<0.05).5.DKK-1 in bone tissue:Compared with the nomal group,the model group was higher(P<0.01);the tripterygium wilfordii tablets group and the high dose group were lower compared with the model group,(P<0.01),the medium dose group was lower(P<0.05)compared with the model group.There was no significant difference between the low dose group(P>0.05)compared with the model group,medium dose group and low dose group were higher compared with the tripterygium wilfordii tablets(P<0.05);?-catenin in bone tissue:Compared with the nomal group,the model group was lower(P<0.01),compared with the model group,the tripterygium wilfordii tablets group,the high dose group,and the medium dose group were higher(P<0.05),and there was no significantly different between the model group and the low dose group(P>0.05);medium dose group and low dose group were lower than tripterygium wilfordii tablets(P<0.05).6.Synovial pathology:The synovial cells of the nomal group were completely arranged,no inflammatory cell infiltration,no neovascularization;model group and low dose rats were the worst;tripterygium wilfordii tablets group,high dose group and medium dose group were relatively better.Conclusion Rheumatoid I can effectively improve weight loss,joint swelling,reduce spleen coefficient,reduce synovial vascular proliferation,decrease serum TNF-? and IL-6 inflammatory factors in AA rats,and down-regulate bone tissue DKK-1.Up-regulation of bone tissue ?-catenin is more effective in the middle and high dose groups,and its mechanism may be achieved by activating the Wnt/?-catenin pathway. |
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