The Relationship Between The Resistance And Virulence Factors In Klebsiella Pneumoniae

Objective:Klebsiella pneumoniae(KP)is one of the important pathogens in nosocomial infections,and the number and drug resistance are increasing,which brings great difficulties in clinical treatment.The purpose of the paper is to investigate the resistance,prevalence,and virulence factor distribution of Klebsiella pneumoniae isolated in clinical,so as to provide the evidence for reasonableMethods:The phoenix-100 microbial identification drug sensitivity analyzer was used to identify Klebsiella pneumoniae isolated from clinical specimens and to detect antimicrobial susceptibility.The mucus phenotype was determined by wire drawing test,and matrix-assisted laser desorption ionization was applied.Rapid identification and homology analysis by time-of-flight mass spectrometry(MAS-MS),using Polymerase Chain Reaction(PCR)to detect related virulence Genes(fimH,entB,ybtA,allS,mrkD,wabG,ureA,uge),PCR positive amplification products were sent to Shanghai Platinum Biotechnology Company for gene sequencing.The relevant data was processed and analyzed by chi-square test and t-test.Results:(1)ICU and sputum samples were the most isolated KP.The highest resistance rate was piperacillin,52.5%,and the lowest resistance rate was amikacin 25.3%.The high mucus strain was used for common antibiotics.The drug resistance rate was not high,which was significantly lower than that of the non-mucus phenotype strain,and the difference was statistically significant.The most serious department in which CRKP occurs is the ICU.(2)80 strains of KP isolated in our hospital were identified by K.pneumoniae by mass spectrometry,and the scores were all>2.0.According to the analysis of ClinProTools 3.0 software,the protein peak information of the strains is roughly the same.The ICU 23 KP strains have a certain degree of homology,the closely related strains,or the patients separated from the same bed number,or the ICUs separated from the same period.patient.(3)The detection rate of ureA in virulence gene detection was the highest,accounting for 90%of the total;secondly,the positive rate of both fimH and entB was 88.8%;the total virulence gene deletion was allS,accounting for 12.5%of the total;No positive strain was detected in the wabG gene.The positive expression rate of allS and uge genes in highly viscous strains was significantly higher than that in non-viscous strains,and the difference was statistically significant.The virulence gene was detected by the six gene co-detection patterns:the positive rate of simultaneous expression of fimH+entB+ybtA+mrkD+ureA+uge was 38.8%;in the model carrying 7 virulence genes simultaneously,the positive rate of highly viscous strains To be significantly higher than the non-viscous strain,it is indicated that the highly viscous strain carries more virulence genes than the non-viscous strain.Conclusions:Klebsiella pneumoniae has the highest incidence in ICU and respiratory specimens.Highly viscous strains are more sensitive to antibiotics than non-sticky strains,but carry more virulence genes,which should cause clinical attention.It is suggested that Klebsiella pneumoniae may cause the loss of some virulence genes in the process of drug resistance.