Changes Of Monocytes And Their Subsets In Primary Sj(?)gren's Syndrome

Objective:To further analyze pSS by detecting the ratio of monocytes and their subtypes(CD14++CD16-,CD14++CD16+,CD14+CD16++)in peripheral blood of normal subjects and patients with primary Sj(?)gren's syndrome(pSS).The relationship between the number of monocytes and their subtypes in patients with disease activity index(SSDAI)and erythrocyte sedimentation rate(ESR)and C-reactive protein(CRP)to explore the changes of monocytes and their subpopulations in primary Sj(?)gren's syndrome.Methods:A total of 19 healthy controls(HC)and 16 patients with pSS were enrolled in this study.The percentage of peripheral blood mononuclear cells and their subtypes were detected by flow cytometry(CD14++CD16-,CD14++CD16+,CD14).+CD16++)ratio and analysis of the correlation between monocytes and their CD14++CD16+ isoforms and ESR,CRP,and pSS disease activity index(SSDAI).Results:1.The proportion of monocytes in the peripheral blood of patients withpSS(0.66±0.13,n=16)was significantly higher than that of the healthy control group(0.57 ± 0.02,n=19),the difference was statistically significant(p <0.05).The results of monocyte subset detection showed that the proportion of CD14++CD16+ subpopulation in peripheral blood of patients with pSS(58.34±5.7,n=16])was higher than that of healthy controls(19.47±5.66,n=19)significantly increased,the difference was statistically significant(p < 0.001);the proportion of CD14 +CD16-subpopulation in peripheral blood of patients with pSS(16.78 ±4.45,n = 16)compared with healthy control group(79.04 ± 5.66),n=19)significantly decreased,the difference was statistically significant(p <0.001).The proportion of CD14+CD16++ subpopulation in peripheral blood of patients with pSS(13.97±3.06,n=16)was not significantly different from that of healthy controls(12.88 ± 2.79,n=19)(p=0.429>0.05).2.There was no correlation between peripheral blood mononuclear cells and SSDAI scores in patients with pSS(r=0.251,p>0.05);no correlation with ESR(r=0.254,p>0.05);no correlation with CRP(r=0.209,p>0.05);BUT CD14++CD16+ monocyte subsets were positively correlated with SSEDAI score(r=0.942,p<0.001);positively correlated with ESR(r=0.974,p<0.001);positively correlated with CRP(r=0.961),p <0.001).Conclusion:The expression level of CD14++CD16+ in peripheral blood mononuclear cells and monocyte subsets of pSS patients was significantly higher than that of healthy controls,while the expression level of CD14++CD16-monocyte subsets was lower than that of healthy controls;in pSS patients The expression level of CD14++CD16+subgroup in monocytes was positively correlated with SSDAI score and ESR and CRP levels,but there was no significant correlation between monocyte and SSDAI scores and ESR and CRP levels.