| Objective:This study was to investigate the status of Ureaplasma urealyticum(Uu)infection and its biovars and serotypes in pregnant women with premature rupture of membranes;to investigate the correlation between different pregnancy times,the number of abortions and the Uu positive rate in patients with premature rupture of membranes;to analyze the sensitivity of Uu infection to antibiotics,and provide experimental evidence for clinical treatment of Uu infection during pregnancy and prevention of premature rupture of membranes.Methods:1.According to the inclusion criteria,171 pregnant women with premature rupture of membranes were selected as the observation group,100 healthy pregnant women as the control group,and 86 healthy non-pregnant women as the blank group.Record basic patient information.2.Collected the subjects’cervical secretions and vaginal secretions.Uu was cultured with Uu liquid culture identification and drug sensitivity kit,and the specimens with positive Uu liquid culture concentration greater than 10~4(CCU/ml)and their drug susceptibility were recorded.Microscopic examination of vaginal secretions for screening for trichomoniasis,Candida and Gardnerella infections.3.The liquid culture-positive specimens were subjected to nucleic acid extraction,and the Uu multi-band antigen(MBA)conserved fragments were designed to design clusters and fractionated primers.The positive PCR samples were amplified by conventional PCR method;the amplified products were condensed by 2%agarose.Gel electrophoresis detection.4.Use SPSS19.0 statistical software for data processing.The measurement data were average±standard deviation,and the count data was expressed in frequency.The comparison between the measurement data groups was performed by an independent sample t test,and the count data was mainly analyzed by chi-square test,P<0.05 difference was statistically significant.Results:1.The overall positive rate of Uu in pregnant women with premature rupture of membranes was 75.44%(129/171),which was higher than54.0%(54/100)in the control group and 33.33%(26/78)in the blank group.The difference between the three groups was statistically significant(P<0.05).The more number of pregnancies or abortions,the Uu positive rate is higher.There was a statistically significant difference in Uu positive rate between different pregnancy times and abortion times.(P<0.05&P<0.05).2.The positive rate of the Uu biovar 2 in observation group was24.03%(31/129),which was higher than 11.11%(6/54)in the control group and 7.69%(2/26)in the blank group.There were significant differences in the distribution of the two biovars in the three groups(P<0.05).The distribution of Uu serotype 1 and serotype 6 were significantly different among the three groups(P<0.05).There was no significant difference in the distribution of serotype 3 and mixed serotypes,(P>0.05).3.Uu infection is more sensitive to tetracycline antibiotics,all of which are 100%;the sensitivity to quinolones is low,and the sensitivity is less than 20%.The sensitivity of macrolide antibiotics is different,and the sensitivity to azithromycin and clarithromycin is more than 80%.There is no significant difference in the sensitivity of different Uu biovars and serotypes against antibiotics,(P>0.05).Conclusion:1.Uu biovar 2 and serotype 1 are closely related to the occurrence of premature rupture of membranes;2.Uu infection is highly sensitive to tetracycline antibacterial drugs,and can be used as a first-line drug for clinical diagnosis and treatment of Uu infection in the absence of contraindications;macrolide antibiotics are less sensitive than tetracyclines;in addition,quinolone antibiotics are less sensitive and are not recommended for use in empiric therapy.Uu infection in pregnant women can be treated with azithromycin;Uu biovars and serotypes do not affect clinical medication. |
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