Regulatory Mechanism Of Gene Transcriptional Changes In Rat Liver Regeneration

The liver has many important functions such as synthesis,detoxification,and metabolism.The liver has a unique ability to regenerate.Under the induction of physical,chemical and biological factors,the residual hepatocytes rapidly activate and enter the process of the cell cycle and cell proliferation to compensate for the lost liver tissue and restore the biological function.This process is called liver regeneration(LR).Liver regeneration is a complex and precise process controlled by a combination of factors,involving multiple cells and factors,as well as a variety of physiological and biochemical activities.In this thesis,we explored the a series of gene transcription regulations,including gene methylation,miRNA,lncRNA,circRNA,protein phosphorylation,protein acetylation,protein glycosylation,and protein ubiquitination in rat liver regeneration to understand their roles and effects in liver regeneration and to further elucidate the molecular mechanism of rat liver regeneration.In this study,2/3 hepatectomy in SD rats was used as a model to obtain gene methylation,miRNA,lncRNA,circRNA,protein phosphorylation,protein acetylation,and protein glycosylation in rat liver regeneration by high-throughput sequencing.Gene/RNA/protein types and expression changes involved in the regulation of protein ubiquitination,and determination of genes/RNA/proteins related to gene transcription activities,such as GO,NCBI and IPA,biostatistics and computer analysis The ratio values were further screened for differential gene/RNA/protein,and systematic biology to analyze their effects on gene transcription activity in rat liver regeneration.The results showed that the gene transcription activity in rat liver regeneration:(1)involved 307 methylation genes,in which the number of methylation genes in transcription initiation,transcription elongation,transcription termination and post-transcription modification was 106,23,28 and 66;(2)involving 230 miRNAs,wherein the number of miRNAs for transcription initiation,transcription elongation,transcription termination and post-transcriptional modification is 196,19,33 and 138;(3)involving 271 lncRNAs,Among them,the number of lncRNAs in transcription initiation,transcription elongation,transcription termination and post-transcriptional modification are 207,16,0 and 188;(4)195 circRNAs are involved,in which the number of CRCs of transcription initiation,transcription elongation,transcription termination and post-transcriptional modification are 195,124,152 and 193;(5)57 kinds of phosphorylated proteins,wherein transcription The number of phosphorylated proteins in the beginning,transcription elongation,transcription termination and post-transcriptional modification are 18,13,18 and 17;(6)37 kinds of proteins with acetylation modification,in which transcription initiation,transcription extension,and transcription termination And the number of post-transcriptional modified acetylated proteins are 12,2,4,and 12;(7)10 glycosylated proteins,including transcription initiation,transcription elongation,transcription termination,and post-transcriptionally modified sugars.The number of basicized proteins is in the order of 3,0,0 and 3;(8)49 species of ubiquitin-modified proteins,wherein transcription The number of glycosylated proteins in the beginning,transcription elongation,transcription termination and post-transcriptional modification were 13,6,5 and 6;(9)There were 234 kinds of mRNA related to liver regeneration in rats and 64 kinds of proteins involved in each regulation.