The Effect Of Rucaparib On Cervical Cancer And Radiosensitivity

Objective: To investigate the effect of Rucaparib on the inhibition and radiosensitivity of cervical cancer.Methods:(1)CCK-8 and colony formation assay to detect the effect of Rucaparib on the proliferation of cervical cancer cells;(2)Flow cytometry was used to detect the effect of Rucaparib on apoptosis and cycle of cervical cancer cells;(3)Western blot was used to detect the effect of Rucaparib on CDK4 and Cyclin D1 in cervical cancer cells;(4)The effect of Rucaparib combined with radiotherapy on the proliferation of cervical cancer cells was detected by colony formation assay;(5)Flow cytometry was used to detect the effect of Rucaparib combined with radiotherapy on the cell cycle of cervical cancer;(6)Western blot was used to detect the effect of Rucaparib combined with radiotherapy on CDK4 and Cyclin D1 in cervical cancer cells;(7)The expression of DNA damage marker ?-H2 AX was detected by immunofluorescence assay;(8)Establish an animal model of transplanted tumors and observe the inhibitory effect of Rucaparib on cervical cancer;(9)Immunohistochemistry was used to detect the effect of Rucaparib on the proliferation of cervical cancer.Results:(1)With the increase of Rucaparib concentration,the cell viability gradually decreased.When Rucaparib acted on cervical cancer cells at different concentrations,the clone formation ability decreased,and the higher the drug concentration,the lower the clone formation rate;(2)Rucapaprib had no obvious apoptotic effect on cervical cancer cells,but induced G2/M arrest of cervical cancer cells;(3)With the increase of Rucaparib concentration,the expression of CDK4 and Cyclin D1 decreased;(4)Rucaparib combined with radiotherapy can inhibit the colony forming ability of cervical cancer cells,and with the increase of drug concentration,the inhibition of colony forming ability was more obvious;(5)Combination of Rucaparib and radiotherapy promoted G2/M arrest of cervical cancer cells;(6)When Rucaparib was combined with radiotherapy,the expression of CDK4 and Cyclin D1 was significantly lower than that of control group and single-use group;(7)After 2 and 24 hours of combination of Rucaparib and radiotherapy,the positive expression rate of DNA damage marker ?-H2 AX in the combined group was significantly higher than that in the control group and the single group.The combination of Rucaparib and radiotherapy delayed the repair of DNA damage;(8)Tumor volume of Rucaparib group was smaller than that of control group,and there was no significant difference in body weight between the two groups;(9)The expression of Ki-67 protein in Rucaparib group was significantly lower than that in control group.Conclusions: Rucaparib can inhibit the proliferation of cervical cancer in vitro and vivo,induce G2/M phase arrest of cervical cancer cells.Rucaparib can improve radiosensitivity of cervical cancer cells,provide basic experimental basis for the clinical treatment of cervical cancer by Rucaparib,and provide a new choice for radiosensitizer of cervical cancer.