Proteomic Analysis Of Human Breast Cancer Cell Line SKBR-3 Treated With Trichostatin A

ObjectiveTo explore the anti-breast cancer target and pathway of trichostatin A(TSA)in human breast cancer cell line SKBR-3,the proteomic expression profiles of SKBR-3 treated with TSA and control group were analyzed by comparative proteomics.It provides experimental basis for the application of HADC inhibitors in clinical treatment.Methods1.In vitro cultured human breast cancer cell line SKBR-3,was divided into two groups according to the drug treatment: experimental group(n=3)(TSA-treat)SKBR-3 and control group(n = 3)(con)SKBR-3,treated for 48 hours.2.Differential protein analysis and mass spectrometry were carried out between the SKBR-3 treated with TSA and the control group by comparative proteomics based on TMT technique.3.GO(Gene Ontology)software,InterProScan software,Wolfpsort software and KEGG(Kyoto Encyclopedia of Genes and Genomes)database were used for further bioinformatics analysis of the differentially expressed proteins.ResultsA total of 337 differentially expressed proteins,including 169 up-regulated proteins and 168 down-regulated proteins,were screened out according to the up-regulation and down-regulation of the differentially expressed protein(1.3-fold)as the standard(P-value < 0.05).The increased protein is concentrated in the cytoplasmic region and acts on nutrient metabolism and signal transduction processes,With catalytic activity,binding activity and transporter activity;The reduced protein is concentrated in the nucleus region and is used for cell division,proliferation and DNA synthesis,damage repair process,and with catalytic activity?binding activity?transcription activity,and transporter activity? ConclusionsIn this study,a number of differential expression proteins were screened,trichostatin A may play its anti-tumor mechanism through energy metabolism,regulation of cell cycle,signaling pathway and other pathways directly or indirectly acting on the target protein.