Study On The Regulation Of Skeletal Muscle Injury And Repair By Lnc247

The long non-coding RNA is an RNA molecule which is formed by direct transcription of RNA polymerase II and is less than 200 bp and less than 100 kb.The long non-coding RNA Lnc247 found by sequencing of transcriptional group is a natural antisense LncRNA of Foxk1.Foxk1 gene plays an important role in regulating the proliferation and differentiation of skeletal muscle satellite cells.Therefore,the study of the function of Lnc247 can provide a basis for revealing the regulatory role of lncRNA in skeletal muscle injury and repair.In this paper,molecular biology,histochemical,transcriptional group and bioinformatics analysis were used.The effect of Lnc247 on Foxk1,an important transcriptional regulator in skeletal muscle regeneration,and its effect on skeletal muscle injury and repair were studied.1.Using high performance visualization tools,integrated genome browser(Integrative Genomics Viewer IGV)and BLAT functional analysis of UCSC,it is found that some of the 3'UTR regions of Lnc247 and Foxk1 complement each other,indicating that Lnc247 is a natural antisense lncRNA of Foxk1.2.The overexpression of(AAV)in the anterior tibia muscle showed that the protein level of Foxk1 was significantly lower than that of the control group,indicating that the overexpression of Lnc247 had a negative regulatory effect on the expression of Foxk1 gene.3.The results of Western Blot and qPCR showed that in the skeletal muscle injury model,the expression of myogenic differentiation transcription factor MyoD and the expression of e-MHC in the Lnc247 overexpression group were significantly higher than that of the control group.The results showed that the expression of Lnc247 could promote the myogenic differentiation of injured muscle fibers and accelerate the process of skeletal muscle injury and repair.4.The repair of skeletal muscle injury was observed by H & E staining.The results showed that the cross section area of regenerated muscle fibers with central nucleus in Lnc247 overexpression group was larger than that in control group.5.Using transcription group and bioinformatics analysis,GO gene function enrichment analysis and KEGG pathway analysis were performed for differentially expressed genes in Lnc247 overexpression injury group and control injury group.It was found that significant enrichment of GO term resulted in muscle tissue formation,energy metabolism,protein synthesis and metabolism,and significant enrichment of KEGG pathway resulted in citric acid cycle and amino acid biosynthesis,which indicates that skeletal muscle needs more energy and material synthesis to repair the damaged muscle fibers after injury.