| 1.Objective:To study the serum PPARy/ANGPTL receptor ligand levels and ox-LDL levels in patients with phlegm turbidity syndrome of hyperlipidemia,observing the difference between phlegm turbidity syndrome patients and non-phlegm turbidity syndrome patients.The difference provides more biological basis for the clinical diagnosis of patients with phlegm turbidity syndrome of hyperlipidemia.It also detects the levels and the composition ratio of LDL and HDL subtypes,which provide a reference for the diagnosis of phlegm turbidity syndrome of hyperlipidemia.2.Method:2.1 Research methods of serum ANGPTL3,ANGPTL4,PPARy and ox-LDL levels in patients with phlegm turbidity syndrome of hyperlipidemia and hyperlipidemia116 patients with hyperlipidemia who were hospitalized in Guanganmen Hospital from November 2018 to February 2019 were enrolled in the hyperlipidemia group.24 healthy people from the Guanganmen Hospital and the Southern District Medical Center were used as the control group.According to the diagnostic criteria of Chinese medicine,patients with hyperlipidemia were divided into phlegm turbidity syndrome group and non-phlegm turbidity syndrome group.Taking the fasting venous blood of the subject in the morning,the levels of serum ANGPTL3,ANGPTL4,PPARy.and ox-LDL were measured by ELISA.To observe the difference between the indicators of hyperlipidemia and healthy people,phlegm turbidity syndrome group and non-phlegm turbidity syndrome group.2.2 Research methods of serum HDL and LDL subtypes in patients with phlegm turbidity syndrome of hyperlipidemia116 patients with hyperlipidemia who were hospitalized in Guanganmen Hospital from November 2018 to February 2019 were enrolled in the hyperlipidemia group.24 healthy people from the Guanganmen Hospital and the Southern District Medical Center were used as the control group.According to the diagnostic criteria of Chinese medicine,patients with hyperlipidemia were divided into phlegm turbidity syndrome group and non-phlegm turbidity syndrome group.The lipoprotein system was used to detect the levels and the composition ratio of serum HDL and LDL subtypes.To observe the difference between the indicators of hyperlipidemia and healthy people,phlegm turbidity syndrome group and non-phlegm turbidity syndrome group.2.3 Statistical analysisThe statistical software used was spss21.0,the measurement data was expressed as(x ± s),and the t-test was used for comparison between independent groups.The count data is represented by n(%),and the comparison between groups is performed by chi-square analysis.P<0.05 indicates that the difference was statistically,significant.3.Result:3.1 Results of serum ANGPTL3,ANGPTL4,PPAR y,ox-LDL levels in patients with hyperlipidemia3.1.1 Results of blood lipid levels in patients with hyperlipidemia and healthy peopleCompared with the healthy group,the levels of TC,TG,LDL-C,VLDL-C,APOA1,APOB,and LP(a)in the hyperlipidemia group were significantly higher than those in the healthy group(P<0.01).3.1.2 Results of serum ANGPTL3,ANGPTL4,PPAR y,ox-LDL in patients with hyperlipidemiaThe levels of ANGPTL3,ANGPTL4 and ox-LDL in the hyperlipidemia group were significantly higher than those in the healthy group(P<0.01),3.2 Results of serum ANGPTL3,ANGPTL4,PPAR ?,ox-LDL levels in patients with phlegm turbidity syndrome of hyperlipidemia3.2.1 Comparison of blood lipid levels between patients with phlegm turbidity syndrome of hyperlipidemiaCompared with the non-phlegm turbidity syndrome,the levels of TG and VLDL-C in the phlegm turbidity syndrome group were significantly higher(P<0.01),and the levels of HDL-C and APOA1 were significant lower.(P<0.01).3.2.2 Results of serum ANGPTL3,ANGPTL4,PPAR?,ox-LDL levels in patients with phlegm turbidity syndrome of hyperlipidemiaCompared with the non-phlegm turbidity syndrome,the levels of ANGPTL3.ANGPTL4.ox-LDL in the phlegm turbidity syndrome group increased significantly(P<0.01):PPAR ? level decreased significantly(P<0.01).3.3 Results of serum LDL,HDL subtype levels and composition ratio in patients with hyperlipidemia3.3.1 The ratio of HDL subtypes between hyperlipidemia patients and healthy people:Compared with healthy people.HDL1%and Large(1-3)%of hyperlipidemia group were significantly decreased(P<0.01);HDL2%and HDL 3%were decreased(P<0.05);HDL6%increased(P<0.05);HDL7%,HDL8%,HDL9%increased significantly(P<0.01);Small(8-10)%increased(P<0.05).3.3.2 HDL subtype results in patients with hyperlipidemia and healthy people:Compared with the healthy group,the HDL1 in the hyperlipidemia group was significantly lower(P<0.01);the Large(1-3)was decreased(P<0.05);the HDL7 was increased(P<0.05);HDL8,HDL9,and Small(8-10)were significantly increased(P<0.01).3.3.3 The ratio of LDL subtypes in patients with hyperlipidemia and healthy people:Compared with healthy people.LDL1%and LDL2%in hyperlipidemia group were significantly decreased(P<0.01);LDL3%and LDL4%were significantly increased(P<0.01).3.3.4 Low-density lipoprotein subtype levels and average LDL particle size in patients with hyperlipidemia and healthy people:Compared with the healthy group,the LDL1 and mean LDL particle size of hyperlipidemia patients were significantly decreased(P<0.01);LDL3 and LDL4 were significantly increased(P<0.01).3.4 Results of serum LDL,HDL subtype levels and composition ratio in patients with phlegm turbidity syndrome of hyperlipidemia3.4.1 HDL subtype composition ratio of patients with phlegm turbidity syndrome of hyperlipidemia:Compared with the non-phlegm turbidity syndrome group,HDL1%,HDL2%,and Large(1-3)%of the phlegm turbidity syndrome group decreased significantly(P<0.01);HDL4%decreased(P<0.05).HDL8%,HDL9%,HDL10%,and Small(8-10)%were significantly different(P<0.01).3.4.2 HDL subtype level results in patients with hyperlipidemia and phlegm syndrome:Compared with the non-phlegm turbidity group,the HDL1,HDL2,and Large(1-3)of the phlegm turbidity syndrome group were significantly decreased(P<0.01);the HDL4 decreased(P<0.05);HDL8,HDL9,HDL 10,and Small(8-10)were significantly increased(P<0.01).3.4.3 The ratio of LDL subtype composition in patients with phlegm turbidity syndrome of hyperlipidemia:Compared with the non-phlegm turbidity group,the LDL1%and LDL2%of the phlegm turbidity syndrome group decreased significantly(P<0.01);LDL4%increased(P<0.05).3.4.4 Low-density lipoprotein subtype levels and mean LDL particle size in patients with phlegm turbidity syndrome of hyperlipidemia:Compared with the non-phlegm turbidity group,the LDL1 and LDL2 in the phlegm turbidity syndrome group decreased(P<0.01);the average LDL particle size decreased,(P<0.05);LDL5 increased(P<0.05).4.Conclusion:4.1 ANGPTL3.4 levels rise.PPARy levels decrease.ox-LDL levels rise in the phlegm turbidity syndrome of hyperlipidemia.It is suggested that PPAR y/ANGPTLparticipates in the formation of phlegm turbidity syndrome by regulating ox-LDL.4.2 Serum HDL subtypes in patients with phlegm turbidity syndrome ofhyperlipidemia show a trend of large particle level and composition ratio decrease.small particle level and composition ratio increase;Serum LDL subtypes show a trend toward smaller particles.It suggests that small particles of HDL and LDL are involved in the formation of hyperlipidemia phlegm turbidity syndrome,large particles of HDL and LDL may have protective effects on phlegm turbidity syndrome. |
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