Astragaloside ? Alleviates Cardiac Hypertrophy Caused By Abdominal Aortic Constriction In Rats Through Nrf2/HO-1 Pathway

Objective:To investigate the effect and mechanism of Astragaloside ? on cardiac hypertrophy induced by abdominal aorta constriction in rats.Methods:40 SD adult male rats were selected and abdominal aorta constriction models of cardiac hypertrophy were established,which were divided into four groups:model group,benazepril group,high dose Astragaloside ? group and low dose Astragaloside ? group,and another10 rats were selected to establish the sham operation group.In the benazepril group and the low-dose and high-dose Astragaloside ? group,10mg·kg-1·d-1 and 40mg·kg-1·d-1 and 80mg·kg-1·d-1 groups were given benazepril,and the sham group and the model group were given the same volume of normal saline.ELISA was used to detect the changes of BNP level in serum of rats,and the HM/BM and HM/TL were measured.The morphological changes of myocardial cells were detected by immunofluorescence,and the protein expression of Nrf2 and HO-1 in heart tissue were detected by western blot,RTq-PCR was used to detect the expression of Nrf2 and ho-1 mRNA in heart tissue.Results:1.Compared with control group,model group rats LVEDD,LVESD,LVPWD,LVEDP and LVESP at the end of the and the serum BNP levels were significantly elevated,LVEF,LVFS,E/A,+dp/dt_maxax and-dp/dt_maxwere obviously reduced.2.Compared with the model group,the levels of LVEDD,LVESD,LVPWD,LVEDP,LVESP and serum BNP in the benazepril group and the low and high dose Astragaloside ? group were decreased to varying degrees,while the levels of LVEF,LVFS,E/A,+dp/dtmax and-dp/dtmax were increased.3.Compared with benazepril group,LVEDD,LVESD,LVPWD,LVEDP,LVESP and serum BNP levels were increased in the low dose Astragaloside ? group,while LVEF,LVFS,E/A,+dp/dtmax and-dp/dtmax were decreased in the high dose group.4.Compared with the sham group,the heart volume of the model group increased,and HM/TL and HM/BM increased.Compared with the model group,the heart volume of the rats in the benazepril group and the low and high dose Astragaloside ? group decreased,while that of the HM/TL and HM/BM decreased.Compared with the benazepril group,the heart volume of the rats in the low dose Astragaloside ? group increased,while that of the HM/TL and HM/BM increased.There was no significant difference in the above indicators among the rats in the high dose group.5.Immunofluorescence examination showed that the myocardial cells in model group were hypertrophy and disordered in arrangement,while the myocardial cells in the benazepril group and the low and high Astragaloside ? group were reduced and arranged in order,compared with the low dose Astragaloside ? group,the high dose Astragaloside ? group showed more obviously decrease in myocardial cells.6.Compared with the sham group,the expression levels of Nrf2and ho-1 protein and mRNA in the myocardial tissue of the model group were significantly decreased.Compared with the model group,the expression levels of Nrf2 and HO-1 protein and mRNA in the cardiac muscle of the benazepril group and the low and high dose astragaloside iv group were significantly increased.Compared with benazepril group,Nrf2 and ho-1 protein and mRNA expression levels of the rats in the low dose astragaloside iv group were decreased,while those in the high dose group were not obviously different.Conclusion:1.Astragaloside ? can alleviate cardiac hypertrophy induced by abdominal aorta constriction and improve cardiac function;2.Astragaloside ? can alleviate oxidative stress induced by abdominal aorta constriction by activating Nrf2/HO-1 pathway,and play a cardioprotective role.