Construction Of Microbial Colony Growth Model And Evaluation Of Intervention Measures For Automated Endoscope Reprocessor

Objective:According to the status of microbial contamination in the clinical use of AER,a model of AER microbial colony growth was established,and the treatment effect of the intervention on the growth model was evaluated.To ensure the effective reprocessing of AER equipment,further prevent the occurrence of endoscopic secondary pollution,avoid cross-infection between patients and diagnose patients,reduce the outbreak of nosocomial infection caused by AER pollution,and escort the patient's life safety.At the same time,it provides a certain data reference for the development of relevant guidelines in the future.Methods:1.Experimental method:Through the random number table method,two endoscopic automatic cleaning and disinfecting machines of the brand A and B of the digestive endoscopy center of a third-grade hospital in China were selected.Two of the AERs are brand A manufacturers,and the structural design is exactly the same;the other two AERs are from the B manufacturer brand,and the design of the basin and the cover is different from the A manufacturer brand.Similarly,using the method of random number table,4 endoscopes were taken in strict accordance with the inclusion and exclusion criteria.The AER basin and the lid were uniformly applied to the AER basin and the table cover at intervals of 1 hour,4 hours,12 hours,24 hours,48 hours,72 hours and 144 hours.The microbial sampling and culture were carried out on the basin and the table cover respectively.The effects of microbial contamination on the AER basin at different intervals were observed,and the AER microbial contamination model was established.Intervention measures were taken for the AER basin and the canopy to evaluate the effect of the intervention.2.Statistical methods:The measurement data conforms to the normal distribution and is described by the mean?standard deviation.Statistical analysis was performed using the Modified Compertz equation,repeated measures analysis of variance,Pearson chi-square test,and Fisher's exact probability method.The statistical test level?=0.05,P<0.05 was statistically significant.Results:1.Establish a model for the growth of microbial colonies growth in two design types of AER basins and decks.2.In the colony growth of two design types AER basins and canopies,there was a statistically significant difference in colony counts between A design type AER and B design type AER(P<0.05),B design type AER basin and table cover.The colony count was significantly less than the A design type AER.3.In the sampling results of endoscopic biopsy channels after two types of design AER reprocessing,A design type AER and B design type AER were statistically different(P<0.05),while in the results of water vapor channel,the difference was not statistical.Learning meaning.4.As far as the intervention was concerned,the results of colony counts in the intervention group and the control group trough Pearson x~2 were statistically different(P<0.05),which means that the intervention can significantly remove the residues bacteria in the AER basin.Conclusion:1.The mathematical model established in this study can well predict the growth dynamics of the total number of colonies in the basin and the canopy as a function of interval.2.The design structure defects of the AER basin and the cover are important factors leading to the detection of bacteria.There were differences in colony counts between the two design types of AER basins and canopies,and there was no difference in colony counts between AER basins and canopies of the same design type.3.B design type AER basin and table cover colony count is significantly less than A design type AER.Therefore,AER manufacturers should pay attention to AER's own design problems,continuously optimize the structure and function of AER,so that the inside of the basin is immersed in the disinfectant as much as possible.The inner and outer surfaces of the cover are as smooth as possible,in order to obtain a safer,faster and more effective AER.Reduce the incidence of nosocomial infections caused by AER.4.A design type AER has a great difference between the re-treatment endoscopic biopsy channel bacteria detection and the B design type AER,further confirming the defect of the design structure.The sampling results of the biopsy channel can more accurately reflect the effect of AER on endoscopic reprocessing.In clinical practice,the microbial culture of the endoscopic biopsy cavity after retreatment should be strengthened to ensure the safety of the patient's diagnosis and treatment.5.Interventions can significantly remove bacteria remaining in the AER basin and cover.In the actual clinical work,the staff should clean and disinfect the AER basin and the cover after each cleaning and disinfection work,and keep it dry;after the daily diagnosis and treatment work,the part where the disinfectant can not be immersed in the AER The intervention in this study blocked the endoscopic contamination of microorganisms remaining on the AER basin and the lid,and reduced the incidence of nosocomial infection to ensure the safety of endoscopic retreatment.