Expression And Significance Of Merlin,YAP1 And TEAD4 In Ependymal Tumors Of Different Regions Of The Central Nervous System

Objective:The expressions of Merlin,YAP1,TEAD4 and Ki-67 in ependymoma tumor tissues in three different regions of the central nervous system(supratentorial,posterior fossa,and spinal cord)were detected.The difference and correlation of the expression of Merlin,YAP1 and TEAD4 in ependymal tumors in different regions were analyzed,providing experimental basis for further study on the relationship between the Hippo pathway mediated by YAP1 and TEAD4 and Merlin,as well as the difference in prognosis of ependymal tumors in different regions and the selection of therapeutic targets for ependymal tumors.Methods:During the period from January 2012 to December 2018,35 cases of paraffin-embedded specimens of the supratentorial ependymoma were diagnosed in the Department of Pathology,the First Affiliated Hospital of Nanchang University,and 23 cases of posterior cranial fossa tumors were selected,and 40 cases of paraffin specimens after spinal cord ependymoma surgery.The expressions of Merlin,YAP1,TEAD4 and Ki-67 in the three groups of samples were detected by the immunohistochemical method(MaxVision),and the data were statistically analyzed using SPSS24.0 software.Results:1.There was a statistically significant difference in the expression of Merlin in the supratentorial,posterior fossa and spinal ependymoma tumor(?~2=21.388P<0.001).Further,there was a statistically significant difference in the expression of Merlin between the supratentorial ependymoma tumor and the spinal ependymoma tumor(?~2=15.341 P=0.000<0.001);there was a statistically significant difference in the expression of posterior fossa ependymoma tumor and spinal ependymoma tumor(?~2=13.270 P=0.000<0.05);there was no significant difference in the expression between the supratentorial ependymoma tumor and the posterior fossa ependymoma tumor(?~2=0.061 P=0.543>0.05).2.There was no statistical difference in the expression of YAP1 in the supratentorial,posterior fossa and spinal ependymoma tumor(?~2=3.796 P=0.150>0.05).3.There was no statistical difference in the expression of TEAD4 in the supratentorial,posterior fossa and spinal ependymoma tumor(?~2=1.421 P=0.491>0.05).4.In Merlin's different grades of ependymoma tumor,the positive expression rate of ependymoma tumor decreased with the increase of ependymoma tumor grade,while the difference of YAP1 and TEAD4 in different graded ependymoma tumor was not obvious.5.In the supratentorial ependymoma tumor,there was a significant positive correlation between Merlin and YAP1 expression(r=0.431 P=0.01<0.05),and there was a significant positive correlation between YAP1 and TEAD4 expression(r=0.498P=0.002<0.05),but the correlation between Merlin and TEAD4 expression was not statistically significant;in the posterior fossa ependymoma tumor,the correlation between Merlin,YAP1 and TEAD4 expression was not statistically significant;in spinal ependymoma tumor,Merlin was significantly positively correlated with YAP1expression(r=0.352 P=0.026<0.05),but there was no significant correlation between the expression of YAP1 and TEAD4,Merlin and TEAD4.6.The expression of Ki-67 in spinal ependymoma tumor is significantly lower than that of supratentorial and posterior fossa ependymoma tumor.7.There was no correlation between Ki-67 and Merlin,YAP1 and TEAD4expression in the supratentorial,posterior fossa and spinal ependymoma tumor.Conclusion:1.The positive expression rate of Merlin in spinal ependymoma tumor was significantly lower than that in supratentorial ependymoma tumor and posterior fossa ependymoma tumor,which suggesting that Merlin may be a differential protein between spinal cord ependymal tumors and supratentorial and posterior fossa ependymal tumors.2.The expressions of YAP1 and TEAD4 in supratentorial,posterior fossa and spinal ependymal tumors were not statistically different,and the absence of Merlin mainly occurred in the spinal cord,these may be related to the different prognosis of ependymal tumors in different parts.3.The expression rate of proliferation indicator ki-67 in ependymal tumors of spinal cord was significantly lower than that of supratentorial ependymal tumors and posterior fossa ependymal tumors,while the expression of tumor suppressor protein Merlin in spinal cord was low,and its deletion would lead to the activation of Hippo pathway,suggesting that Merlin may have nothing to do with the proliferation of ependymal tumors.