Clinical Application Of CTCs Detection In Patients With Primary Hepatic Carcinoma And Research On Biological Characteristics Of GBS In Neonatal Infection

Chapter Ⅰ Clinical application of CTCs detection in patients with primary hepatic carcinomaObjective:To preliminarily investigate the clinical application value ofthe types of circulating tumor cells(CTCs)on prognosis estimation and recurrence monitoring ofpatients with primary hepatic carcinoma.Methods:67 patients with primaiy hepatic carcinoma diagnosed by Zhujiang hospital from January 2014 to July 2018 were recruited in this study.The correlation of CTCs expression level and patients’ clinical pathological stage,Ki-67 antigen expression level of tumor tissue,serum AFP expression level as well as blood cell parameters was analyzed statistically.Furthermore,the changes of CTCs expression level of 22 patients before and during treatment and their progression ofdiseases were analyzed with time series analysis method.Results:The study results showed total CTCs counts and the percentage of interstitial CTCs of patients with primary hepatic carcinoma were positively correlated with Ki-67 antigen expression level oftumor tissue,while total CTCs counts of patients with primary hepatic carcinoma showed no significant correlation with patients’BCLC stage,serum AFP expression level and blood cell parameters(NLR,PLR and RDW);the results of dynamic monitoring showed 1 to 2 months after hepatectomy,total CTCs counts and the percentage of interstitial CTCs of patients with primary hepatic carcinoma both decreased;total CTCs counts and the percentage ofinterstitial CTCs increased during treatment,indicating the risk of tumor recurrence.Conclusions:The proliferation of tumor tissue could be reflected by CTCs expression level of patients with primary hepatic carcinoma.The dynamic monitoring of CTCs during treatment could contribute to monitor the treatment effect and to detect tumor recurrence early in order to adjust the treatment measures timely and improve prognosis.Chapter Ⅱ Research on biological characteristics of GBS in neonatal infectionObjective:To study the pathogenic characteristics and biological characteristics of clinicalisolated strains of Group B streptococcus(GBS)in neonatal infection,thereby laying the foundation for further research on the pathogenesis of neonatal GBS infection.Methods:Six strains of GBS isolated from infected neonates from March 2014 to December 2016 were enrolled as research subjects.Growth characteristics,anti-phagocytosis characteristics and adhesion characteristics of each strain were measured in the experimental research.Besides,genome-wide sequencing and basic local alignment search tool(BLAST)analysis of each strain were also performed.Results:The difference on growth rate of each strain was found through growth curve.Only the F780 strain got negative result in CAMP test The difference on adhesion rate ofeach strain was found through adhesion test The F780 strain was easier to adhere to Hep-2 cells than other strains.It’s found through phagocytosis test that the phagocytic resistance of F780 strain to RAW264.7 cells was weakest After quantitatively analyzing the interaction of human blood and GBS with flow cytometry,it’s found that the F780 strain was easiest to be phagocytized by human blood phagocytes.It’s found through human whole blood survival test that the 24 hour-survival rate of F780 strain in human whole blood was lowest The results of genome-wide sequencing and BLAST analysis showed cfb,the CAMP factor determining gene of F780 strain,was deleted.Conclusions:According to the results of this study,it’s speculated that the deletion of CAMP factor determining gene cfb may lead to the weakening of anti-phagocytosis ability of GBS strains.This paper provides the scientific basis for the research on anti-phagocytosis mechanism of GBS,which contributes to the research on pathogenesis of GBS in neonatal infection.