Analysis Of 25-hydroxyvitamin D Levels In Infants With Gastrointestinal Cow's Milk Protein Allergy

Cow's milk protein allergy is an abnormal immune response caused by cow's milk protein,which often occurs in infants and young children.Recent studies have found that vitamin D was not only related to calcium-phosphorus metabolism,but also played an important role in immune regulation.In particular,the discovery of vitamin D receptors in body made the research on vitamin D and human immunity become a hot topic.Literature about the relationship between vitamin D and food allergy have been reported at home and abroad,but no coincident conclusion achieved yet.Some studies have pointed out that there's a u-shaped curve relationship between vitamin D and food allergy,which indicated that high or low vitamin D level would lead to increased risk of food allergy,whereas some research have found no correlation between vitamin D and food allergies.Therefore,The relationship between vitamin D and food allergies is inconclusive and deserves further study.This study was designed to analyze the difference of 25-hydroxyvitamin D levels between infants with gastrointestinal cow's milk protein allergic and healthy infants.Objective:1.To analyze whether there is a difference of 25-hydroxyvitamin D levels between gastrointestinal cow's milk protein allergic infants at the first diagnosis and healthy infants.2.To analyze whether there is a difference of 25-hydroxyvitamin D levels between gastrointestinal milk protein allergic infants and healthy infants with the same level of vitamin D supplementation..Methods:1.Object of study The 30 cases of infants who are diagnosed with gastrointestinal cow's milk protein allergy via challenge test at the Boai Hospital from May 2018 to July 2018 were selected as study group,and the 30 healthy infants who underwent physical examination in the department of child health of the hospital at the same time were selected as the control group..The inclusion criteria for both groups are:(1)The age was 1-3 months;(2)the term infants were born in a single fetus with a birth weight of 2.5-4.0 kg,and the mother had no high-risk factors during pregnancy and received routine vitamin D supplementation during pregnancy.(3)artificial feeding(ordinary infant formula)(4)born in Zhongshan,and lived in Zhongshan City;(5)the guardian agreed to let the baby participate in the study and signed an informed consent.Exclusion criteria:(1)congenital metabolic diseases;(2)congenital malformation and abnormal digestive tract structure;(3)in addition to gastrointestinal symptoms,have or suspect other symptoms of systemic allergy;(4)acute infectious disease in the past 1 month.2.Method Both groups of infants were checked blood 25-hydroxyvitamin D level at the time of enrollment.Milk protein allergic infants were fed with amino acid formula or deep hydrolyzed formula,while healthy infants were fed with infant formula.Each group was supplemented with 400UI vitamin D drops(capsule)(Xiamen Xingsha Pharmaceutical,National Pharmaceutical Standard H35021450)daily.In addition,Both groups of infants were engaged in routine outdoor activities and were supplemented with complementary foods.After 3 months of additional vitamin D supplementation,blood samples were taken again to check for 25-hydroxyvitamin D in both groups..25-hydroxy vitamin was detected with electrochemiluminescence immunoassay,using an automatic Electrochemiluminescence immunoassay analyzer(Roche,COBAS E601).Result:1?The 25-hydroxyvitamin D level in the study group and the healthy control group were 74.24±17.26nmol/L and 74.46±14.83nmol/L,respectively.There was no significant difference between the two groups(P>0.05).2?The 25-hydroxyvitamin D level of both groups were increased after taking vitamin D supplements for 3 months,which were up to 123.85±13.93nmol/L and 125.03±10.95nmol/L,respectively.And there was no significant difference between the two groups(P>0.05).(P>0.05).Conclusion:1?There is no difference in the level of 25-hydroxyvitamin D between the infants with gastrointestinal cow's milk protein at the first diagnosis and healthy infants.2?The level of 25-hydroxyvitamin D in gastrointestinal cow's milk protein allergic infants and healthy infants were increased to the same degree after 3 months of vitamin D supplementation.