| Objective:(1)To observe the therapeutic effect of Dingxuan Granule on patients with posterior circulation ischemic vertigo with wind-phlegm-blood stasis syndrome;(2)To explore the effect of Dingxuan Granule on the changes of serum monoamine neurotransmitters in patients with posterior circulation ischemic vertigo with wind-phlegm-blood stasis syndrome before and after treatment;(3)To observe the weight change of rats with posterior circulation ischemia;(4)To explore the effect of Dingxuan Granule on blood circulation ischemia To study the mechanism of Dingxuan Granule in improving posterior circulation ischemia by listing the effects of amine neurotransmitters.Methods:(1)Clinical research:60 patients with wind-phlegm-blood stasis syndrome of PCIV were randomly divided into treatment group and control group.The treatment group was treated with Dingxuan Granule orally,and the control group was treated with Betastine Mesylate Tablets for one week.The scores of TCM syndromes were evaluated before and after treatment,and the content of monoamine neurotransmitters in patients’serum was determined.(2)The experimental research:40 healthy SD rats were selected.After the balance training before operation reached the standard,10 rats were randomly selected and exposed to bilateral vertebral artery surgery as sham operation group.The remaining 30 rats were randomly divided into three groups after occlusion of bilateral vertebral artery:traditional Chinese medicine group,western medicine group and model group.Chinese medicine group was treated with Dingxuan Granule,western medicine group was treated with Betahistine Mesylate Tablet,model group and sham operation group were given saline 5 ml/day intragastric administration,body weight was measured before and 1 week after operation,serum of rats was taken one week after treatment to detect the content of monoamine neurotransmitters in serum.Results:(1)Clinical research:The general data of the two groups were similar and comparable.The average age of patients with PCIV in the two groups was about 70 years old;in the control group,there were 8 males and 22 females;in the treatment group,there were 9 males and 21 females,and the number of females was significantly higher than that of males;in the patients with previous history of cerebral infarction and hypertension,followed by coronary heart disease,there was no significant difference in the combined diseases between the two groups(P>0.05).Before treatment,there was no significant difference in the scores of TCM syndromes between the two groups(P>0.05),and the two groups were comparable.After treatment,the scores of TCM syndromes in the two groups were significantly lower than those before treatment,and the difference was statistically significant(P=0.00);the decrease of TCM syndromes in the treatment group was more obvious than that in the control group,and the difference was statistically significant(P=0.044<0.05).The total effective rate of TCM syndrome treatment in treatment group was higher than that in control group,but the difference was not statistically significant(P>0.05).Serological indicators:Before treatment,the levels of monoamine neurotransmitters in the two groups were similar,but there was no statistical difference.Compared with the control group,the monoamine neurotransmitters in the two groups decreased significantly after treatment(P<0.05);the NE content in the treatment group was significantly lower than that in the control group,while the E,DA and 5-HT content had no significant difference between the t.wo groups(P>0.05).(2)Experimental part:Weight comparison:Before operation,there was no significant difference in body weight between the two groups(P>0.05).On the 7th day after operation,the weight of rats in sham operation group,western medicine group and traditional Chinese medicine group was significantly higher than that in operation group(P<0.05),and the weight of rats in sham operation group was significantly higher than that in traditional Chinese medicine group and Western medicine group(P<0.05).There was no significant difference in body weight between western medicine group and traditional Chinese medicine group(P>0.05).Compared with the preoperative group,the rats in the operation group decreased significantly on the 7th day after operation(P<0.05),while those in the sham operation group,western medicine group and traditional Chinese medicine group increased significantly on the 7th day after operation(P<0.05).Serological indicators:Compared with the operation group,the serological indicators of the sham-operation group,betahistine group and Dingxuan granule group decreased significantly,with statistical difference(P<0.05);while the serological indicators of the Xuan granule group and betahistine group had no significant difference(P>0.05).Conclusion:(1)Dingxuan Granule can significantly improve the symptoms and signs of patients with posterior circulation ischemic vertigo and wind-phlegm-blood stasis syndrome,and improve the quality of life of patients.(2)Dingxuan Granule has definite therapeutic effect on rats with posterior circulation ischemia and can significantly improve the symptoms of weight loss in rats.(3)Dingxuan Granule in the treatment of PCI is related to the reduction of monoamine neurotransmitter content,calcium influx,free radicals and excitatory amino acids release in vivo.It provides new ideas and methods for further exploring the traditional Chinese medicine treatment of PCIV. |
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