Protective Effect Of Hypothermia On Photoreceptor Injury In Mice

Object: To study the protective effect of hypothermia on retinal light injury in mice and its mechanismMethods: 1.A mouse retinal light injury model was established: white fluorescent lamp(LED)was used as the light source,with an average illumination intensity of 12000 Lux and a duration of 12 hours,and f-ERG was measured on the fifth day after illumination.2.Establish a mouse hypothermia model: Place the mice in a constant temperature refrigerator at 8 ° C for 3 hours.Use a thermometer to measure the anal temperature of the mouse immediately after the cold treatment.The anal temperature represents the core temperature of the mouse.3.To test the visual electrophysiology of mice: On the fifth day of the experiment,the binocular f-ERG of four groups of mice was tested.The experimental grouping and treatment were as follows: Thirty-two healthy C-57 male black rats(8 in each group)were randomly divided into 4 groups: normal group,light group,hypothermia group,light-hypothermial group.Mice in the normal group were raised in the normal environment.On the first day of the experiment,the light group and the light-hypothermia group were placed under the light intensity of 12000 Lux for 12 h.In experiment 1?2?3 days,hypothermia and ligth-hypothermia group mice would be exposed to 8 ° C temperature refrigerator and time for 3 hours.4.Detection of retinal protein by Western blot: retinal protein was extracted from four groups of animals,and the expressions of CIRBP and Parp were measured.5.Retinal thickness measurement: the thickness of each layer of retinal tissue was measured by preparing eye tissue sections of mice.Result: 1.Under the condition of light damage,compared with the light group,the amplitude of b-wave and a-wave of f-ERG in the light-hypothermia group was significantly increased,and the difference was statistically significant(p < 0.05).2.Western blot was used to detect the protein content in retinal tissue.Compared with the light group,the expression of CIRBP increased and the expression of Parp decreased.,and the difference was statistically significant.3.Retinal tissue section showed that compared with the light group,the thickness of ONL and PS layers in the light hypothermic group increased.Conclusion: 1.Low temperature has an anti-light damage effect in the retina of mice.2.In the retinas of animals,hypothermia can fight against retinal light damage by over-activating the expression of cold shock protein and inhibiting the over-expression of Parp.3.In retinal tissue sections,hypothermia prevents the reduction of the thickness of the layers of the retina caused by photodamage,especially the PS layer and the ONL layer.