Expression Of TAP68 In In Bone Marrow Mononuclear Cells Of Patients With Multiple Myeloma And Clinical Significance

BackgroundMultiple myeloma(MM)is a malignant hematological neoplastic disease caused by malignant proliferation of plasma cells in bone marrow.The incidence of malignant tumors accounted for 1% of all malignant tumors,ranking second in hematological malignancies.It is characterized by malignant clonal proliferation of plasma cells in bone marrow,secreting a large number of monoclonal immunoglobulin,A series of clinical manifestations such as bone destruction,anemia,renal function injury,hypercalcemia,bacterial virus infection and hyperviscosity syndrome.In recent years,with the wide application of autologous hematopoietic stem cell transplantation,cellular immunotherapy,proteasome inhibitors and new immunomo dulators,the long-term survival of patients with multiple myeloma has been significantly improved.However,due to the extremely changeable biological behavior of MM,its pathogenesis is not fully understood,and MM cells are prone to multi-drug resistance against cancer drugs,so MM patients will eventually develop drug resistance and recurrence leading to treatment failure and death.Therefore,it is particularly important to further study the mechanism of the occurrence and development of MM and improve the survival rate of patients.TAP68 is a telomere binding protein associated protein,which is homologous to the centrosome protein Pcp1 of Schizosaccharomyces cerevisiae and belongs to the superprotein family of SMC domain.Studies have shown that the biological function of TAP68 is highly related to cell cycle.The loss of function or abnormal expression of TAP68 will directly lead to centrosome abnormality and chromosome instability.In addition,overexpression of TAP68 can regulate telomere length by affecting telomerase activity of tumor cells,suggesting that TAP68 may be a key molecule in regulating mitosis and telomere regulation of malignant tumor cells.In order to further clarify the biological function of TAP68 in hematopoietic malignant tumors,the expression of TAP68 in multiple myeloma and its correlation with clinical data were studied.It lays a foundation for further study on the expression regulation and mechanism of TAP68 in myeloma.Objective:To investigate the expression of TAP68 in multiple myeloma and its correlation with the prognosis of patients with multiple myeloma.Methods:25 patients with multiple myeloma were selected as the study group,and 10 patients with normal bone marrow were selected as the control group.The patients in the study group were treated with bortezomib combined with dexamethasone and thalidomide for 4 courses.Bone marrow mononuclear cells were isolated from bone marrow samples of control group and study group before and after treatment,and the expression of TAP68 in bone marrow mononuclear cells was detected by real-time fluorescence quantitative detection and Western Blot.To compare the expression of TAP68 in the study group and the control group.Collect the data of the experiment was collected to analysis.Results:1.The relative expression of TAP68 in BMMCs of study group was significantly higher than that of control group(P < 0 05),and the expression of TAP68 in BMMCs of MM patients was significantly decreased after treatment(P < 0 05).2.According to the international staging system established by the International Myeloma working Group,there was significant difference in the expression of TAP68 in BMMCs of MM patients with different stages(P < 0.05).TAP68 was positively correlated with M protein(r=0.693,P=0.031)and ? 2-microglobulin(?2-MG)(r=0.458,P=0.037).The expression of TAP68 protein was not correlated with sex,age and immunophenotype of MM patients(P > 0.05).Conclusion:In this study,real-time immunofluorescence quantitative(qPCR)and Western blot(Western blot)were used to detect the expression of TAP68 in multiple myeloma mononuclear cells,and the relationship between the expression of TAP68 and clinical data was analyzed.The results are as follows:(1)The expression of TAP68 in BMMCs of MM patients was significantly increased,which was related to clinical stage,M protein and ? 2-MG.(2)TAP68 may play an important role in the occurrence and development of multiple myeloma.