Repair Of Radioactive Damage Of Mice Skin By Adipose-derived Stem Cells

BackgroundRadiation therapy is a method of cancer treatment.It uses high doses of radiation to kill cancer cells and prevent them from spreading.About half of cancer patients receive radiation therapy.With the development of radiotherapy,the number of diseases that can be used for radiotherapy has gradually increased.As the scope of treatment continues to expand,skin damage caused by radiation has also received increasing attention.During radiotherapy,normal surrounding tissues are also damaged,leading to complications such as skin atrophy,soft tissue fibrosis,defoliation,epithelial ulcers,and even poor wound healing.Irradiated wounds are usually resistant to conventional treatment and may often require surgical reconstruction interventions.Reconstruction options usually include skin grafts and local flaps,but complications due to radiation are significantly increased.The damage caused by radiation therapy not only affects the patient's physical health,but also affects the patient's mental health and productivity.Therefore,it is a major problem for patients and remains a major challenge in modern medicine.Bioactive dressings,bioengineered skin,etc.have been used for the treatment of radiation skin damage.Adipose-Derived Stem Cells(ADSCs)are widely found in various organs of the body.Cells are widely distributed,abundant in source,and easy to obtain.They can be isolated and cultivated by certain methods,and have strong proliferative ability.They have become one of the main sources of seed cells.It is expected to provide new treatments for the treatment of radiation damage.PurposeIn this experiment,the effects of ADSCs on skin radiation damage of mice were studied.The changes of inflammatory cells,neovascularization and fibroblasts in the skin HE staining were compared.Then we compare the number,density and arrangement of elastic fibers in Masson staining after ADSCs treatment.To investigate the therapeutic effect of ADSCs on skin injury by radiotherapy.It provides a new idea for the clinical treatment of skin radiation injury.Method1.C57BL/6 mouse inguinal and renal perirenal adipose tissue was extracted and isolated by collagen enzyme method to obtain ADSCs.2.The ADSCs obtained were identified and the surface markers of CD 44,CD 45,CD 31 and CD 90.2 by flow cytometry.3.The osteogenic differentiation ability of ADSCs was identified and 21 days were induced by osteogenic medium.4.The fat-induced differentiation ability of ADSCs was identified,and the fat-induced medium was used for 14 days.5.Using a linear accelerator to irradiate the skin of C57BL/6 mice,a mouse skin RD model was prepared.6.After 2 weeks of local injection of ADSCs,the number of inflammatory cells,neovascularization and fibroblasts in different experimental groups were counted and compared.7.After 2 weeks of local injection of ADSCs,Masson staining was used to compare collagen density and arrangement.Result1.The surface markers of ADSCs in isolated mice were CD 44 positive,CD 45 positive,CD 31 low expression and CD 90.2 positive.2.The isolated and extracted mice ADSCs had good osteogenesis and lipogenic differentiation.3.The number of neovascularization and fibroblasts was significantly higher in the experimental group than in the control group.4.The number of inflammatory cells in the experimental group was significantly lower than that in the control group.5.Collagen density increased obviously in the experimental group and collagen was arranged more neatly than in the control group.Conclusion 1.Local subcutaneous injection of ADSCs can promote epidermal cell proliferation.2.ADSCs local subcutaneous injection can accelerate the inflammatory response,promote angiogenesis.3.The local subcutaneous injection of ADSCs can promote the proliferation of fibroblasts.4.The local subcutaneous injection of ADSCs can promote collagen proliferation.