| Objective: Recent studies have shown that zinc-band domain 1 contains a protein encoded by two zinc-band domains,recently cloned from the human HLA locus and involved in transcriptional reg?Lation,and may play a potential mediating role in m?Ltiple biological processes,including m?Ltidrug resistance,tumorigenesis,and cell cycle.In this study,by detecting Single nucleotide polymorphism of ZNRD1 gene rs3869068,rs16896970 and rs1048412,the correlation between ZNRD1 gene and the risk of HIV-1 infection and the correlation between CD4+T cell changes before and after antiviral treatment in guangxi pop?Lation were discussed.Methods:A total of 369 subjects were included in this study,including 237 cases in the case group and 132 cases in the healthy control group.The gene polymorphisms at three loci,ZNRD1 rs3869068,rs16896970 and rs1048412,were detected using the SnaPshot method.The samples were verified by gene sequencing with the Sanger method,and the distribution frequencies of alleles and genotypes were calc?Lated.The comparison of three Single nucleotide polymorphism(SNP)sites by 2 test was performed to determine whether the control group and each case group were from the same Mendelian group,and whether they were representative and comparable.The Odds ratio(OR)and 95% confidence interval(CI)were calc?Lated after adjusting for age and gender by unconditioned Logistic regression analysis,and the relationship between genetic polymorphism of three SNP sites and genetic susceptibility to HIV-1 infection was analyzed,as well as the correlation between changes in CD4+T cells before and after antiviral treatment.Results:1.The genotypes of the three polymorphisms of ZNRD1 gene in the control group and HIV-1 group(rs3869068,rs16896970 and rs1048412)were consistent with the Hardy-Weinberg balance.The test samples selected in this study were comparable and representative.2.There were CC,CT and TT genotypes at rs3869068.Compared with the control group,there was no statistical difference between the carrying frequency of ZNRD1 gene rs3869068 genotype CT,TT,allele T,dominant model CT+TT,and recessive model CT+CC and the risk of HIV-1 infection(all P values were greater than 0.05).After logistic regression analysis,there was no statistical difference between age and gender.There was also no association between the risk of HIV-1 infection in the control group and the sex stratified comparison.3.There were AA,AG,and GG genotypes at rs16896970.Compared with the control group,the carrying frequencies of AG,GG,allele G,dominant model AG+GG of ZNRD1 gene at rs16896970 co?Ld reduce the risk of HIV-1 infection(all P values were less than 0.05).Recessive models(AA+AG)also increased the risk of HIV-1 infection.In the male pop?Lation,ZNRD1 rs16896970 mutant heterozygous AG,mutant homozygous GG,allele G,and dominant model AG+GG reduced the risk of HIV-1 infection,while recessive model AA+AG increased the risk of HIV-1 infection(P < 0.05).In the female pop?Lation,there were no differences in ZNRD1 rs16896970 site mutation heterozygote AG,mutant homozygote GG,allele G,dominant model AG+GG,and recessive model AA+AG(all P values were greater than 0.05).4.There were AA,AG and GG genotypes at rs1048412.Compared with the control group,there was no statistical difference between the carrying frequency of ZNRD1 gene at rs1048412,AG,GG,allele A,AG+GG in the combined model and AG+AA in the invisible model and the risk of HIV-1 infection(all P values were greater than 0.05).After logistic regression analysis,there was no statistical difference between age and gender.There was also no association between the risk of HIV-1 infection in the control group and the sex stratified comparison.5.To analyze the influence of three SNPS,including ZNRD1 gene rs3869068,rs16896970 and rs1048412,on the changes of CD4+T cells in HIV-1 patients after treatment.At rs3869068,CC,CT and TT genotypes were not correlated with CD4+T cells in HIV-1 patients after treatment(all P values were greater than 0.05).At rs16896970,there was no correlation between AA,AG,GG genotypes and CD4+T cells in HIV-1 infected patients after treatment(all P values were greater than 0.05).At rs1048412,there was no correlation between AA,AG,GG genotypes and CD4+T cells in HIV-1 infected patients after treatment(all P values were greater than 0.05).6.The susceptibility of patients with HIV-1 infection to three SNPS genotypes,namely,rs3869068,rs16896970 and rs1048412,was analyzed at different baseline levels of CD4+T cells.The OR value and 95%cl of genotypes and alleles for the disease were calc?Lated by non-conditional Logistic regression analysis and adjusted for gender and age.The res?Lts showed that at rs3869068 and rs16896970,there was no correlation between the genotype and allele and the level of baseline CD4(all P values were greater than 0.05).At rs1048412,compared with AA genotype,AG genotype and A allele were significantly different from baseline CD4(<100)group(all P values were less than 0.05).Conclusion :1.ZNRD1 rs16896970 locus mutation heterozygote AG,mutant homozygote GG,allele G,dominant model AG+GG can reduce the risk of HIV-1 infection,while recessive model AA+AG increases the risk of HIV-1 infection.2.The polymorphisms of ZNRD1rs3869068 and rs1048412 have no significant correlation with the risk of HIV-1infection.3.In male pop?Lation,ZNRD1rs16896970 site mutation heterozygote AG,mutant homozygote GG,allele G,and dominant model AG+GG can reduce the risk of HIV-1 infection,while recessive model AA+AG increases the risk of HIV-1 infection.4.Gene polymorphisms of three ZNRD1 genes,rs3869068,rs16896970 and rs1048412,were not significantly correlated with CD4+T cell level after treatment.5.ZNRD1 rs1048412 was significantly correlated with the baseline CD4+T cell level,and the baseline CD4+T cell level was high and the overall immune function was good in HIV-1 patients with AG genotype and G allele before treatment.6.There was no significant correlation between ZNRD1rs3869068 and rs16896970 and baseline CD4+T cell level. |
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