Effect Of Xinfeng Capsule On Expression Level And Mechanism Of Peripheral Blood Syk And VEGF In RA Patients

1 purpose Fresh air Capsule(Xinfeng Capsule,XFC)treatment of Rheumatoid arthritis(Rheumatoid arthritis,RA)in patients with curative effect,the expression of Syk,VEGF,HIF-1 alpha level changes,the change of cell inflammatory factors and related laboratory index,study the influence of the new wind Capsule in patients with RA,peripheral blood Syk,VEGF expression level and the correlation of RA patients,and to further discuss the mechanism analysis of new wind Capsule in patients with RA.2 methods 2.1 theoretical analysis Through a lot of literature retrieval and delve into,the paper mainly discusses the analysis of RA and peripheral blood Syk,VEGF,HIF-1 alpha and disease activity,and the relationship between the prognosis,and to explore the relationship between the RA patients with spleen deficiency of traditional Chinese medicine,analysis to clarify medical etiology and pathogenesis and inner link,RA patients treated from the spleen theory research progress.2.2 clinical study 2.2.1 changes and correlation of Syk,VEGF,hif-1 and other laboratory indexes in peripheral blood of RA patients: Healthy controls from anhui province hospital physical examination center to select 20 cases of normal physical examination,the course of the disease,the general data contrast has no obvious difference of the age,by using enzyme-linked immunosorbent(ELISA)to detect the TNF alpha healthy controls and patients with RA,IL-4,IL-6,IL-10 levels,adopt fully automatic biochemical analyzer,healthy controls and patients with RA of RF,CRP,ESR,CCP,C3,C4 and Ig M and Ig A,Ig G,horizontal;Automatic blood sedimentation analyzer was used to detect ESR level.The statistical software was SPSS25.0.2.2.2 air capsule in patients with RA clinical curative effect,life quality and lab index changes: selection in August 2017 to August 2018 in anhui province hospital hospital li rheumatism lead of 60 cases of RA patients,adopts the method of random grouping,divided into two group to fluorine,and fresh air capsules,each 30 cases,consistent with ACR in 1987 and 2010,which was jointly launched by ACR/EULAR classification of RA diagnosis standard,two groups of course,the general data contrast has no statistical significance of the age,according to the patient the ESR,VAS score and joint swelling pain symptoms,DAS28 score calculation.In the form of questionnaire,the quality of life and TCM symptoms of selected patients were observed for scoring.3.Results 3.1 study on the changes and correlation of serum indexes in RA patients The expression levels of Syk,VEGF and hif-1 in RA group were significantly higher than those in healthy group(P< 0.01).The expression levels of cytokines TNF-and il-6 in the RA group were significantly higher than those in the healthy group(P<0.05),and the expression levels of il-4 and il-10 were significantly lower than those in the healthy group(P<0.05).The levels of RF,ccp-ab,ESR,CRP,Ig G and C3 in the RA group were significantly higher than those in the healthy group(P<0.05).Syk and VEGF levels were positively correlated with RF,CCP,ESR and DAS-28 levels,and HIF-1 levels were positively correlated with RF and DAS-28 levels.Level of TNF alpha RA patients with RF,CCP and positively related to the level of CRP,DAS-28 IL-4 levels and RF,CCP,ESR,DAS-28 level negative correlation(P < 0.05),IL-10 level and RF negative correlation,CCP,DAS-28 level,level of IL-6 and RF,CCP,Ig G,are positively related to the level of the ESR,DAS-28 Syk and TNF alpha,further analysis the RA group are positively related to the level of IL-6 and IL-4,IL-10 negative correlation;VEGF was positively correlated with TNF-and il-6,and negatively correlated with il-4 and il-10.Hif-1 was positively correlated with TNF-and il-6,and negatively correlated with il-4 and il-10.Syk and VEGF levels were positively correlated with joint swelling in RA patients(P<0.05).Joint tenderness was positively correlated with Syk level(P<0.05)and negatively correlated with hif-1 level(P<0.05).Anorexia was positively correlated with the level of Syk and VEGF(P<0.05),and negatively correlated with the level of hif-1(P<0.05).There was a positive correlation between the level of Syk and abdominal distension(P<0.05).There was a negative correlation between the level of Syk and VEGF(P<0.05).In RA group,psychological function,H A Q level were positively correlated with peripheral blood Syk,VEGF and hif-1 level(P<0.05).Healthy self-knowledge was negatively correlated with hif-1 level(P<0.05).These results suggest that peripheral blood Syk,VEGF and hif-1 levels are also correlated with the living weight of RA patients.3.2.Clinical effect of xinfeng capsule on RA patients After diagnosis and treatment,the total effective rate of patients in both groups was 86.6% in XFC group and 80.0% in LEF group.Compared with LEF group,the total effective rate of patients in XFC group was significantly better than that in LEF group(P<0.05).3.3 effects of XFC on Syk,VEGF and hif-1 levels in RA patients There was no significant difference in the levels of Syk,VEGF and hif-1 between the two groups of RA patients before treatment(P>0.05),and the levels of Syk,VEGF and hif-1 in the two groups were improved after treatment.XFC group was significantly better than LEF group(P<0.05).3.4 effects of XFC on TNF-,il-4,il-6 and il-10 levels in RA patients There was no significant difference in the levels of TNF-,il-4,il-6 and il-10 between RA patients in the two groups before treatment(P>0.05).The results of TNF-and il-6 in the two groups after treatment were significantly lower than those before treatment(P<0.05),and the mean values of il-4 and il-10 were significantly higher in the two groups after treatment(P<0.01).Compared with LEF group,TNF-and il-6 levels decreased more significantly in XCF group(P<0.05).Il-4 and il-10 levels were significantly increased(P<0.05).3.5 the effect of XFC on the changes of immune laboratory indicators in RA patients Compared with before treatment,RF,CCP,Ig G,Ig A,Ig M,ESR,CRP,C3,C4 and DAS-28 scores in XFC group were significantly decreased(P<0.05),while RF,CCP-AB,ESR,CRP,C3,C4 and DAS-28 scores in LEF group were significantly decreased(P<0.05).Compared with LEF group,RF,CCPB,ESR,CRP and DAS-28 scores in XFC group decreased significantly(P<0.05).3.6 influence of XFC on TCM syndrome points in RA patients After treatment,the symptoms of joint tenderness and morning stiffness of RA patients in the two groups were significantly improved(P<0.05),but there was no significant difference between the two groups in treatment(P>0.05).After treatment,the symptoms of joint heaviness,joint swelling,joint pain,tiredness,lack of breath and speech,loss of appetite,loose stool in both groups were alleviated,and compared with LEF group,XFC group was significantly alleviated(P <0.05).3.7 influence of XFC on quality of life indicators of RA patients There was no significant difference in the quality of life between RA patients in the two groups before treatment(P>0.05),and the quality of life in the two groups after treatment was better than before treatment.In addition to psychological function and healthy self-knowledge,the effect of XFC group was significantly better than that of LEF group(P<0.05).4.Conclusion 4.1.Theoretical studies show that: 4.1.1 The incidence of RA patients is related to the expression levels of Syk and VEGF in peripheral blood;4.1.2 The expression of Syk,VEGF and related cytokines in peripheral blood of patients with RA is closely related to traditional Chinese medicine spleen deficiency4.1.3 Treatment from the spleen is an important way to treat patients with RA;Jianpi Huashi Tongluo method is the main treatment principle of rheumatoid arthritis.4.2 Analysis of the Expression Levels,Inflammatory Factors and Correlation of Peripheral Blood Syk and VEGF in RA Patients The expression levels of Syk,VEGF,HIF-1? in RA group were significantly higher than that of normal people;The expression levels of cytokines TNF-? and IL-6 in the RA group were higher than that of normal people,and the expression levels of IL-4 and IL-10 were lower than that of normal people.Syk,VEGF levels in RA patients were positively correlated with RF,CCP,ESR,and DAS-28 levels,and HIF-1? water averages were positively correlated with RF and DAS-28 levels.It is suggested that peripheral blood Syk,VEGF,HIF-1? are related to disease activity,and the higher the disease activity,the higher the level of expression of Syk,VEGF,and HIF-1?;Syk is positively correlated with TNF-? and IL-6 levels and negatively correlated with IL-4 and IL-10;VEGF is positively related to TNF-? and IL-6,and is negatively related to IL-4 and IL-10;HIF-1? is positively related to TNF-? and IL-6 levels,and is negatively related to IL-4 and IL-10.It is suggested that the levels of peripheral blood Syk,VEGF,HIF-1? have some intrinsic relationships with inflammatory factors TNF-?,IL-4,IL-6,IL-10;And they are closely related to TCM syndromes,quality of life and symptoms of spleen deficiency.4.3.Effect of Xinfeng Capsule on the Clinical Therapeutic Effect and Indices of RA Patients 4.3.1 Xinfeng Capsule is effective in treating RA,which can control the patient's condition and significantly reduce disease activity;Can improve the patient's joint swelling,morning stiffness,spleen deficiency and other symptoms,regulate the patient's mental health,improve the patient's quality of life 4.3.2 Xinfeng Capsule can reduce the levels of peripheral blood Syk,VEGF,HIF-1?,decrease the levels of IL-6,TNF-?,and increase the levels of IL-4 and IL-10 in RA patients.4.4.Possible Mechanism of Xinfeng Capsule for the Treatment of RA Patients 4.4.1 Xinfeng capsule inhibits the progression of inflammation by regulating the level of cytokines;4.4.2 Xinfeng capsule can lower the expression levels of Syk,VEGF,HIF-1?and reduce the incidence of disease;4.4.3 Xinfeng Capsule may balance the level of the cell network by regulating Syk,VEGF,HIF-1?and cytokines,thereby improving the joint and inflammatory symptoms of RA patients.