Expression And Its Significance Of MiR-320c,msi-2 In Patients With Acute Leukemia

Objective:The purpose of this study is to detect the expression levels of miR-320c and musashi-2?msi-2?in adult acute leukemia?AL?patients of different types and different treatment periods,and to explore the relationship between their expression levels and the occurrence,development and prognosis of leukemia.It is expected to provide new methods and laboratory basis for clinical diagnosis,treatment and prognosis evaluation of AL.Methods:1.Research subjects:A total of 150 patients with acute leukemia?Acute leukemia,AL?were selected from January 2017 to January 2018 in the Department of Hematology,the first affiliated Hospital of Gannan Medical College,There were46 patients with acute lymphoblastic leukemia?ALL?and 104 patients with acute non-lymphocytic leukemia?ANLL?.The experimental group was divided into newly diagnosed group,refractory relapse group and remission group according to different periods of disease treatment.The control group consisted of 20 patients with non-malignant hematological diseases admitted to the hospital at the same time.Bone marrow samples from all experimental and control groups were collected at the same time.2.Research methods:Bone marrow mononuclear cells were separated by separation solution.The expression levels of miR-320c and msi-2 mRNA in bone marrow mononuclear cells were detected by real-time quantitative PCR?RT-qPCR?.The expression level of msi-2 protein in bone marrow mononuclear cells was detected by Western blot?WB?.3.Statistical methods:The data was analyzed using SPSS18.0 statistical software.Mann-Whitney U rank sum test was used to compare the expression levels of miR-320c and msi-2 between groups.Spearman rank correlation analysis was performed between miR-320c and msi-2 expression levels and clinical data.The survival analysis was performed by Kaplan-Meier method,and the survival rate was compared by log-rank test.P<0.05 was considered statistically significant.Results:1.The expression of miR-320c in the newly diagnosed group and refractory recurrence group was significantly different from that in the control group?P=0.047,P=0.01?;There was no significant difference between the remission group and the control group?P=0.676?;The expression of miR-320c in the newly diagnosed group and refractory recurrence group was significantly different from that in the remission group?P=0.000,P=0.000?;There was no significant difference between the newly diagnosed group and the refractory recurrence group?P=0.911?.The expression of msi-2 mRNA in the newly diagnosed group and refractory recurrence group was significantly different from that in the control group?P=0.015,P=0.001?;There was no significant difference between the remission group and the control group?P=0.629?;There was a statistically significant difference between the initial treatment group,the refractory relapse group and the remission group?P=0.009,P=0.000?.There was no significant difference between the initial treatment group and the refractory relapse group?P=0.076?;The expression of the msi-2 protein in the four groups was consistent with the msi-2 mRNA expression.2.There was no significant difference in the expression of miR-320c between the newly diagnosed ALL and ANLL?P=0.891?;There was no significant difference in the expression of miR-320c in the newly diagnosed subtypes of ANLL?P>0.05?.There was no significant difference in the expression of msi-2 mRNA between the newly diagnosed ALL and ANLL?P=0.062?;There was no significant difference in the expression of msi-2 mRNA between the newly diagnosed ANLL subtypes?P>0.05?;The expression of msi-2 protein in ALL and ANLL was consistent with the expression of msi-2 mRNA.3.The expression levels of miR-320c gene and msi-2 mRNA in patients with newly diagnosed AL were not associated with gender,age and bone marrow NPM1gene?P>0.05?,There was no correlation between miR-320c and peripheral blood WBC,HGB,PLT,LDH,TAM,WT1 gene,ferritin and bone marrow immature cells?P>0.05?,but it was negatively correlated with the expression of?₂ microglobulin??₂-MG?in peripheral blood?P<0.05?.There was no correlation between msi-2mRNA and peripheral blood WBC,HGB,LDH,TAM,?2-MG,WT1 gene,ferritin and bone marrow immature cells?P>0.05?,but it was positively correlated with peripheral blood PLT count?P<0.05?.4.There was no significant difference in the expression of miR-320c in the complete remission group?CR group?compared with the non-complete remission group?NR group??P=0.881?.There was no significant difference in the expression of msi-2 in the CR group compared with the NR group?P=0.923?.There was no correlation between the expression of miR-320c gene and msi-2 mRNA in AL?r=-0.170,P=0.361?.5.There was no significant difference in the survival rate between the miR-320c high expression group and the low expression group?P>0.05?.Conclusion:1.The expression level of miR-320c can provide a basis for the diagnosis and assessment of disease status in AL patients.2.The expression level of msi-2 can provide a basis for the diagnosis and assessment of disease status in AL patients.