| BACKGROUND:Colorectal cancer is a common malignant tumor of digestive system which seriously threatens human health in the world.In recent years,with the rise of people’s living standards,the incidence of colorectal cancer has been increasing year by year,and it is becoming younger and younger,which poses a serious threat to human health and life.CYLD,also known as familial cytoskeletal protein,was named by Kovalenko et al.in 2003 for the first time in familial cylindrical tumor disease(also known as "head scalp tumor syndrome").It has been widely studied in the scientific community because of its tumor suppressing function.IL-8 is a member of the CXC chemokine family and is secreted by immune cells and tumor cells.It has the functions of adhesion,directed migration and activation of cytotoxic activity,so it plays an important role in the process of inflammation.Studies have shown that IL-8 is also associated with tumor progression and metastasis.However,the combined detection of CYLD and IL-8 expression in colorectal cancer and precancerous lesions has not been studied in depth.Whether the expression of CYLD and IL-8 in colorectal cancer is correlated is still unclear.In this study,we evaluated the possibility of combined detection of two biological molecules as early predictors of colorectal cancer by detecting changes in the expression of CYLD and IL-8 in precancerous lesions and colorectal cancer,in the hope that it would improve early the diagnosis rate of colorectal cancer.PURPOSE:The expression of CYLD and IL-8 in colorectal cancer and precancerous lesions were examined to explore the correlation between the two.METHODS:From June 2017 to September 2018,81 specimens of colorectal tissues were collected from Huaihe Hospital of Henan University,which were diagnosed as colorectal cancer and resected surgically.The specimens of colorectal mucosa biopsy obtained by the endoscopy center of our hospital through fibrocolonoscopy included 30 cases of high-grade intraepithelial neoplasia,30 cases of low-grade intraepithelial neoplasia and 80 cases of normal colonic tissue.A total of 221 cases of colorectal tissue,including 159 colon specimens and 62 rectal specimens,were detected by immunohistochemistry to detect the expression of CYLD and IL-8 in different tissues.Statistical analysis was performed using SPSS 24.0 statistical software.The comparison between positive grades was performed by nonparametric test.Spearman correlation analysis was used to analyze the correlation between CYLD and IL-8 expression in colorectal cancer.In addition,the relationship between the expression and clinical pathological data(age,gender,tumor diameter,tumor location,depth of invasion,lymph node metastasis,clinical TNM stage,CEA,Ki-67 index)was analyzed retrospectively.RESULTS:The expression of CYLD in colorectal cancer group,high grade intraepithelial neoplasia group and low grade intraepithelial neoplasia group was lower than that in normal mucosa group.The positive expression rate of CYLD in normal colorectal mucosa group(87.5%),low-grade intraepithelial neoplasia group(80.0%),high-grade intraepithelial neoplasia group(66.7%)and colorectal cancer group(65.4%)were compared.The expression of CYLD decreased gradually from normal mucosa to colorectal cancer cells.There was no significant difference in the expression of CYLD between colorectal cancer group and high-grade intraepithelial neoplasia group(P=0.513),but there was significant difference in the expression of CYLD between other groups(P<0.05).The expression of IL-8 in low-grade intraepithelial neoplasia,high-grade intraepithelial neoplasia and colorectal cancer was higher than that in normal tissues.The positive expression rate of IL-8 in normal colorectal mucosa group(35.0%)< low grade intraepithelial neoplasia group(80.0%)< high grade intraepithelial neoplasia group(90.0%)< colorectal cancer group(96.2%).The expression of IL-8 increased gradually from normal mucosa to colorectal cancer cells.There was no significant difference in the expression of IL-8 between colorectal cancer group and high-grade intraepithelial neoplasia group(P=0.504),and there was significant difference in the expression of IL-8 between other groups(P<0.05).The expression of CYLD and IL-8 in colorectal cancer was correlated with clinicopathological data(age,gender,tumor diameter,tumor location,depth of invasion,lymph node metastasis,clinical TNM stage,CEA,Ki-67 index).Statistical results showed that the expression of CYLD was closely related to clinical TNM staging(P < 0.001),depth of invasion(P < 0.001),lymph node metastasis(P < 0.001),tumor diameter(P < 0.001),Ki-67 index(P < 0.001),and the difference was statistically significant(P < 0.05).The expression of CYLD in clinical TNM stage III-IV tissue specimens was lower than that in stage I-II tissue specimens.The expression of CYLD in tissues with lymph node metastasis was lower than that in tissues without lymph node metastasis.The expression of CYLD was lower in T3-T4 stage than in T1-T2 stage.The expression of CYLD in tissues with tumor diameter(≥5 cm)was lower than that in tissues with tumor diameter(< 5 cm).The expression of CYLD in tissues with Ki-67 index(≥50%)was lower than that in tissues with Ki-67 index(< 50%).That is,the closer the clinical TNM stage is to the advanced stage,the deeper the infiltration depth is,the earlier the lymph node metastasis is,the larger the diameter of the tumor is,and the higher the Ki-67 index is,the lower the expression level of CYLD is.The expression of IL-8 was closely correlated with clinical TNM stage(P < 0.001),depth of invasion(P < 0.001),lymph node metastasis(P < 0.001),tumor diameter(P = 0.001),Ki-67 index(P < 0.001),and the difference was statistically significant(P < 0.05).The expression of IL-8 in clinical stage III-IV tissue specimens was higher than that in stage I-II tissue specimens.The expression of IL-8 in tissues with lymph node metastasis was higher than that in tissues without lymph node metastasis.The expression of IL-8 in T3-T4 tissue specimens was higher than that in T1-T2 tissue specimens.The expression of IL-8 in tissues with tumor diameter(≥5 cm)was higher than that in tissues with tumor diameter(< 5 cm).The expression of IL-8 in tissues with Ki-67 index(≥50%)was higher than that in tissues with Ki-67 index(< 50%).That is,the closer the clinical stage is to the advanced stage,the deeper the infiltration depth is,the earlier the lymph node metastasis is,the larger the diameter of the tumor is,and the higher the Ki67 index is,the higher the expression level of IL-8 is.At the same time,the expression of CYLD and IL-8 was not significantly correlated with gender,age,tumor location and CEA value,and the results showed no statistical difference(P < 0.05).The expression of CYLD and IL-8 in colorectal cancer tissues was analyzed.The results showed that there was a negative correlation between CYLD and IL-8 expression in colorectal cancer tissues(P<0.001,r=-0.677).CONCLUSION:1.Inhibition of CYLD expression may lead to the occurrence of early colorectal cancer,and may promote the progression of colorectal cancer,indicate poor prognosis as well.2.IL-8 may promote the occurrence of early colorectal cancer,and promote the progress of colorectal cancer,and is associated with poor prognosis.3.Inhibition of CYLD may promote the production of IL-8,and thus promote the progress of colorectal cancer. |
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