| Objective:Recently,studies in domestic and foreign populations have shown that HLA-B alleles may be associated with drug rash caused by different antiepileptic drugs,but there is no such report in Anhui Han population.The aim of this study was to investigate the association between HLA-B genes and severe drug eruptions induced by antiepileptic drugs in Anhui Han population,and to understand the immunological pathogenesis of severe drug eruptions.Methods:A case-control study was conducted to collect 25 antiepileptic drugs-induced severe drug eruption patients and 50 tolerant control blood samples in Anhui,respectively.The enrolled groups were all Han population.HLA-B genotype was detected by sequence-specific primer-polymerase chain reaction?PCR-SSP?,and PLINK1.07 software was used to analyze the difference in HLA-B allele frequency between the patient group and the tolerant control group.Results:1)In drug-specific analysis,the carrier rate of HLA-B*15:02 was significantly higher in antiepileptic drugs compared with antiepileptic drugs-tolerant controls(P=9.6×10-6;OR=10.92;95%CI=3.07-41.00,especially in carbamazepine-severe cutaneous adverse reactions case(P=7.0×10-6;OR=36;95%CI=4.65-375.97).Similar associations in phenobarbital were seen for groups with severe cutaneous adverse reactions case compared with drugs-tolerant controls groups?P=0.013;OR=45;95%CI=1.47-7827.55?.No associations between severe cutaneous adverse reactions case to drugs-tolerant controls groups were observed for the lamotrigine?P=0.879;OR=0.33;95%CI=0.01-8.01 2?Compared with drug-tolerant controls,the carrier rates of HLA-B*40:01 was lower in cases of induced by AEDs?p=0.045;OR=0.18;95%CI=0.03-0.97?.Conclusions:In conclusion,we confirmed the strong association between HLA-B*15:02 and carbamazepine-severe cutaneous adverse reactions in Anhui Province.HLA-B*40:01allele may be a protective factor in severe cutaneous adverse reactions. |
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