Analysis Of Risk Factors For New Bone Formation In Patients With Ankylosing Spondylitis And Its Research With 14-3-3? Antibody

objectiveTo investigate the influencing factors of new bone formation and the correlation with 14-3-3antibody in patients with ankylosing spondylitis(AS).MethodsA total of 223 patients with AS were enrolled.The clinical and laboratory parameters of AS patients were recorded in detail.The disease activity indicators and functional indicators were calculated.The sacroiliacjoints and spine radiographs were taken and the modified Stoke AS spine score was calculated.(m SASSS),sacroiliac joint magnetic resonance scan,comparing the level of m SASSS,spinal involvement(m SASSS>0)and bone bridge formation rate between groups.Serum 14-3-3? antibody concentrations were measured by ELASA method in 180 AS patients,90 RA patients,and 90 normal controls.The differences in 14-3-3? antibody positive rates and 14-3-3? antibody concentration levels between different groups were compared.ResultsSerum 14-3-3? antibody levels were different in 180 patients with AS,90 patients with RA,and 90 normal controls(x2=9.663,P=0.008).The positive rate of serum 14-3-3?antibody in the three groups was Significant difference(x2=6.013,P=0.043).The m SASSS values ?(P=0.004)and smoking(P=0.043)were significantly different between the 14-3-3? antibody negative and positive groups.Age,duration of disease,time of misdiagnosis,X-ray stage,disease activity index were not found different between the 14-3-3? antibody negative and positive groups(P>0.05).The misdiagnosis time ? 5 years,age ? 40 years,and the active disease group in which m SASSS significantly higher than the misdiagnosis time <5 years,age <40 years and disease activity general group(P<0.01),In smoking group,m SASSS? The concentration of14-3-3? antibody was significantly higher than that in the non-smoker group(P<0.01,P=0.043).m SASSS ?bridge formation rate were significant difference in MRI groups and the X-ray groups(P<0.05).Correlation results showed that there was a positive correlation between m SASSS and age,duration of disease,time of misdiagnosis,disease activity index and X-ray groups(P<0.05).Logistics regression analysis showed that antibody 14-3-3? concentration(P<0.0001),disease course(P=0.003),smoking(P=0.006)and sacroiliac joint X-ray staging(P=0.0021)were Risk factors for spinal bone bridges of AS patients.The results of ROC analysis showed that the AUC of the course of disease--the bone bridge was 0.672(P<0.0001),the cut-off value of the disease course was 6.5;the AUC of the concentration of antibody 14-3-3?-the bone bridge was 0.706(P<0.0001),The 14-3-3? cut-off value is 47.76.ConclusionSpinal involvement in AS patients is associated with disease course,smoking,and disease activity.The formation of bone bridge is closely related to the course of disease,smoking,and14-3-3? antibody concentration.