Experimental Study Of Dual-Mode Magnetic Resonance Imaged Magnetic Nanoparticle Delivery System For Cancer Diagnosis And Treatment

Objective Today,The diagnosis and treatment of cancer has always been one of the hotspots in medical research,and MRI is a powerful means of tumor diagnosis.Nanotherapy has become a hot topic today.By compounding MnSiO₃ and Fe₃O₄ and designing it into a tumor microenvironment?TME?responsive degradable system,it can be separated after entering the tumor entity to reduce the contrast effect caused by direct contact between the two,further strengthening MR.Imaging effect.And the drug cisplatin?CDDP?is loaded in the pores of the nanosystems,and the cancer is released after entering the acidic environment of the tumor.Provide research foundation for future clinical applications.Methods A Hollow MnSiO3 nanoparticles were synthesized from mesoporous silica nanoparticles?MSN?with good dispersibility.Then,Fe₃O₄ nanoparticles were modified on the surface of MnSiO₃,then surface aminated?MFN?and grafted polyethylene glycol?MFNP?,and then cisplatin?CDDP?was loaded onto the nanoplatform?MFNP@CDDP?.Physicochemical properties were studied by scanning electron microscopy,thermogravimetry,nitrogen adsorption-desorption experiments,and biocompatibility and cell killing were studied by CCK-8.Cellular uptake and ROS effects were studied by laser confocal and flow cytometry.The subcutaneous tumor-bearing model of nude mice was established to study the distribution and anticancer effects of the nano drug-loading system.Results The PH-responsive nano drug-loading system MFNP@CDDP was successfully constructed.In the acidic environment of the tumor,Fe₃O₄ was separated from the nanosystem,resulting in a Fenton-like reaction,followed by the release of internal CDDP to kill cancer cells,Mn ions.The release and Fe₃O₄ enhance the contrast of MR.Cytological experiments found that MFNP has good biocompatibility to normal somatic cells,while MFNP@CDDP has a killing effect on tumor cells and is concentration dependent.The difference was statistically significant?P<0.05?.The subcutaneous tumor formation model of nude mice was found to have a tumor volume order of Saline>MFNP>CDDP>MFNP@CDDP after injection into the nano drug-loading system.However,except for the CDDP group,the weight of the other three groups of nude mice did not change much.Academic significance?P<0.05?.Conclusion MFNP has good biocompatibility and enhanced MR dual-mode contrast.After loading the drug,MFNP@CDDP can release the drug in the acidic environment of the tumor and effectively treat cancer.The design concept of the integration of diagnosis and treatment has been completed,and new ideas have been provided without the diagnosis and treatment of cancer in the post-clinical.