Correlation Analysis Of Bifidobacterium And Butyrate-producing Bacteria

BackgroundIn recent years,the impact of gut microbiota on human health and disease has gained much attention.The amount of gut microbiota is large yet they have distinct functions,participating in modulating the complex physiological process of the body.Bifidobacterium is a common probiotics and has great impact on the physiology of the body.Soluble dietary fiber can not be digested by the intestine and selectively boost the growth of Bifidobacterium and Lactobacillus.However,the influence of soluble dietary fiber on other intestinal microbes remains unknown.Previous studies about soluble dietary fiber are mostly based on non-sequencing technology,evidence from High-throughput sequencing methods and metabolomics are still inadequate.Objectives1.To analyze the correlation between Bifidobacterium and butyrate-producing bacteria based on the data from large-scale sample of Netherlands and Guangdong province;2.To investigate the effects of FOS and inulin on gut microbiota structure and metabolites in Chinese healthy populations,and explore the effects of soluble dietary fiber on other butyrate-producing bacteria now that it can boost the growth of Bifidobacterium and further verify the correlation between them.Methods1.To select population from 14 districts of Guangdong province by PPS sampling method,collecting background data including age,sex and BMI and so on from 6376 samples,and attained gut microbiota data by Illumina high-throughput sequencing,and conducted correlation analysis between Bifidobacterium and butyrate-producing bacteria based on large-scale population by comparing with the data from Netherlands.2.Participants were randomly divided into FOS group and inulin group and were given FOS and inulin 15g/day for 4 weeks respectively for soluble dietary fiber intervention.Fecal sampling were performed at the beginning and the fourth week.16S rRNA sequencing,flow-cytometer FISH,1H-NMR metabolic profiling were applied and QIIME,LefSe,Spearman analysis were performed to analyze the influence of soluble dietary fiber on gut microbiota and metabolites of healthy population.ResultsAnalysis of gut microbiota from Netherlands and Guangdong province population revealed that the richness of Bifidobacterium negatively correlated with Roseburia,Oscillospira,Odoribacter and some other butyrate-producing bacteria.4 week intervention of FOS and inulin remarkably increased Bifidobacterium and significantly change the overall richness of intestinal flora.The result of sequencing and Flow-Fish revealed that 4 week intake of FOS and inulin markedly reduced Roseburia,Feacalibaterium,Phascolarctobacterium and Ruminococcus.In addition,the supplement of FOS and inulin resulted in increased level of lactate and decreased level of propionate and butyrate in the feces of participants.Correlation analysis concludes that Eubacterium,Fecaelibacterium,and Roseburia was positively correlated with short-chain fatty acids(SCFAs).Furthermore,the decreased level of SCFAs and butyrate-producing bacteria was negatively correlated with the accumulation of lactate.ConclusionsAnalysis of gut microbiota from Netherlands and Guangdong province population revealed that the richness of Bifidobacterium negatively correlated with the richness of some butyrate-producing bacteria.Supplementation of soluble dietary fiber for 4 weeks led to marked changed composition and richness of intestinal microbiota of healthy participants,remarkably boosted the growth of Bifidobacterium but suppressed the growth of butyrate-producing bacteria.In the meanwhile,the level of lactate in the feces increased while the level of propionate and butyrate decreased,which was concurred with the changes of gut microbiota.The decrease of level of SCFAs and butyrate-producing bacteria was negatively correlated with the accumulation of lactate,which further confirmed the adverse trending between them,the involving mechanism warrants further investigation.