Preliminary Study On The Relationship Between The Expression Of Marker Proteins Of Epithelial-mesenchymal Transition And Blood Serum Metabolites Of Colorectal Cancer

Objective:The expression of E-cadherin and N-cadherin in colorectal cancer tissues was determined by immunohistochemical technique and its clinical significance was analyzed.The relationship between epithelial-mesenchymal transition and serum metabolites in colorectal cancer was preliminarily investigated.Attempts to establish a risk scoring model using differential metabolites and clinicopathological data to explore independent risk factors for epithelial-mesenchymal transition in colorectal cancer.Method:1.Immunohistochemical technique was used to detect the expression of E-cadherin and N-cadherin in 100 patients.Patients with epithelial-mesenchymal transition,ie,patients with low expression of E-cadherin and high expression of N-cadherin,are listed as a group.Patients without epithelial-mesenchymal transition were divided into another group.The relationship between the two groups of patients and their clinicopathological parameters was analyzed and observed.2.The chromatogram of the metabolites of serum samples was analyzed by liquid chromatography-mass spectrometry to obtain the original data,and then the statistical analysis software was used for data analysis to screen out the differential metabolites between the groups.3.Screening for independent risk factors associated with epithelial-mesenchymal transition using single-factor,multivariate logistic regression.Results:1.Immunohistochemistry was used to detect the expression of E-cadherin and N-cadherin in 100 cases of cancer tissues.It was found that 20 cases had epithelial-mesenchymal transition and 80 cases had no epithelial-mesenchymal transition.2.Patients with epithelial-mesenchymal transition were associated with tumor lymph node metastasis status,and the difference was statistically significant(p<0.05).It was not related to age,sex,tumor size,differentiation degree,T stage,etc.,and the difference was not statistically significant.Meaning(p>0.05).3.In the total plasma ion chromatogram obtained by Peakview software,differences in serum metabolites between the two groups of patients can be observed;after PCA(principal component analysis)by Markerview software,the two groups of patients can be well separated;112 differential serum metabolites were obtained by t test(P < 0.05).After further discriminating and analyzing using the BRB-ArrayTools software,nine kinds of products with a contribution rate of 100% were obtained.After cluster analysis of these 9 metabolites,it was found that they could distinguish between the two groups of patients.4.Univariate logistic regression was performed on 9 differential metabolites and clinical and pathological data such as gender,age,tumor size,degree of differentiation,and lymph node metastasis.The results showed: 8 differential metabolites and 1 clinical pathological data(lymph node metastasis status))associated with epithelial mesenchymal transition in colorectal cancer.Two independent risk factors associated with epithelial-mesenchymal transition in colorectal cancer were screened by multivariate logistic regression.Conclusions:1.There is a correlation between epithelial-mesenchymal transition and lymph node metastasis status in colorectal cancer;2.There are differential metabolites in the serum of patients with colorectal cancer with epithelial-mesenchymal transition and no epithelial-mesenchymal transition.These differential metabolites are associated with epithelial-mesenchymal transition in colorectal cancer;3.Sphinganine and LysoPC(20:0/0:0)are independent risk factors for epithelial-mesenchymal transition in colorectal cancer.