| ObjectiveProstate cancer is one of the most common malignant tumors in the male genitourinary system and poses a serious threat to human health.Early diagnosis can greatly improve the survival rate of patients.Because of the low level of tumor markers in the early stages of cancer,the detection method requires good specificity and high sensitivity.Electrochemical biosensors have many advantages such as high sensitivity,good selectivity,low price,and simple operation.In recent years,they have received extensive attention from researchers.The key to construct an electrochemical biosensor is to analyze the construction of the interface,and the composition of the interface plays an important role in the sensitivity of the sensor.The popularity of nanomaterials offers endless possibilities for the construction of biosensors.In this paper,a novel composite nanomaterial was first synthesized.Then the new nanomaterial was applied to the construction of electrochemical biosensor.The above sensor was used to detect prostate specific antigen(PSA),which has excellent linear range,low detection limit,good selectivity and fast response.MethodsThe MoS2/GO/o-MWNTs nanocomposite was prepared by hydrothermal synthesis,and the composite had a three-dimensional flower-like nanostructure.The Au nanoparticles were modified by in-situ chemical deposition on MoS2/GO/o-MWNTs composite nanomaterials.The morphology and properties of the composites were analyzed by TEM,FESEM,XPS,XRD and other characterizations.Subsequently,the composite nano-material is dripped on the surface of the cleaned glassy carbon electrode,and the PSA in the bottom liquid is detected by the specific affinity between the antigen and antibody.If the PSA antigen is present in the bottom liquid,the subsequent SA-Ru will be It is assembled to the surface of the electrode,and the emission intensity of Ru is detected by the ECL detection technique,and the concentration of PSA is further known.Results1.The MoS2/GO/o-MWNTs nanocomposites were synthesized by hydrothermal synthesis.The composites were characterized by TEM and FESEM.The composites were found to have a three-dimensional flower structure.Subsequently,gold nanoparticles were deposited by in-situ chemical deposition.Deposited on the flower-like layer of the composite,FESEM shows that the gold nanoparticles have a particle size of about 10 nm and are uniformly dispersed on the three-dimensional flower structure.XPS and XRD results show that the three-dimensional composite nanomaterial has a standard MoS2 hexagonal structure and high purity of materials without other impurities.2.The above composite nanomaterials are applied to an electrochemiluminescence immunosensor,and the optimal experimental conditions are selected.The assembly process was followed by cyclic voltammetry and electrochemical impedance spectroscopy,and the ESA detection technique was used to detect the PSA in the bottom liquid more sensitively.The methodology established in this study showed high sensitivity,good selectivity and stability and could detect the target antigen with timesaving and simple.Conclusions1.The MoS2/GO/o-MWNTs/Au nanocomposites with three-dimensional flower structure were synthesized by hydrothermal synthesis and in-situ chemical deposition.The composite not only has a large specific surface area,but also has improved conductivity and water solubility due to the introduction of GO and o-MWNTs.The gold nanoparticles are deposited on the three-dimensional flower-like layer of the composite material,which can increase the material.The biocompatibility makes the antigen antibodies immobilized on its surface more stable and more numerous.2.The composite nanomaterial was used to construct an electrochemiluminescence immunosensor,and the PSA in the bottom liquid was tested.The experimental results show that the sensor has excellent linear range,low detection limit,good selectivity and fast response.The test results are satisfactory,and it is expected to be used on a large scale in clinical tests in the future. |
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