| PurposeAt present,gestational diabetes mellitus(GDM)is one of the important diseases affecting pregnancy outcome.The increase of blood glucose during pregnancy will lead to a series of adverse pregnancy outcomes:macrosomia,neonatal hypoglycemia,and increased cesarean section.At present,the treatment of gestational diabetes mainly relies on insulin intervention,but it cannot treat gestational diabetes from the pathogenesis.In recent years,biological treatment of diseases has attracted more and more attention,especially related research on mesenchymal stem cells.Therefore,in order to be able to use mesenchymal stem cells for the treatment of gestational diabetes,basic research is particularly urgent.Therefore,we used a mouse model of gestational diabetes to study whether human placenta-derived mesenchymal stem cells(hPDMSCs)can improve blood glucose levels and morphological changes of islet cells after intervention..methodAll placenta in this experiment were derived from human placental tissue delivered during normal full-term cesarean delivery.Mesenchymal stem cells were extracted from the placenta and isolated,cultured and identified.Morphological characteristics of human placenta-derived mesenchymal stem cells observed under an inverted microscopeThe gestational diabetes model was prepared by "high-fat high-sugar diet +low-dose STZ intraperitoneal injection".All modeled mouse models were randomly grouped.Among them,10 normal pregnant mice(group A),10 non-intervention group(group B),10 normalized saline intervention group(group C),and GDM mouse placenta-derived stem cell intervention group(group D)Ten rats were then treated with human placenta-derived mesenchymal stem cells,and the control group was given the same amount of physiological saline.All rats were tested for fasting blood glucose on the 6th,12th and 18th day of pregnancy.On the 18th day of pregnancy,the pancreatic tissues of each group were dissected and paraffin sections were taken.The morphological changes of islet cells in each group were observed under microscope..result1.The human placenta-derived mesenchymal stem cells isolated and obtained were spindle-shaped adherent growth,and the growth state was good.The expression of surface antigen was detected by flow cytometry,and positive expression of CD44,CD105,CD1172.Comparison of blood glucose between GDM group and normal pregnant mouse group(group A):The blood glucose of GDM group was higher than that of normal pregnant mice on the 6th,12th and 18th day of pregnancy,the difference was statistically significant(P<0.05).GDM group comparison:There was no significant difference in blood glucose levels between the non-intervention group(group B)and the saline intervention group(group C)(P>0.05);compared with groups B and C,hPDMSCs The blood glucose level of the intervention group(group D)decreased,the difference was statistically significant(P<0.05);3.Fasting blood glucose changes at different time points in each group:From the 4th day of pregnancy,the blood glucose level of GDM group was stable;the fasting blood glucose of human placenta-derived mesenchymal stem cells intervention group showed a downward trend from the 6th day of pregnancy.4.The islet tissue of GDM rats in each group had different degrees of damage,islet cell necrosis,islet edge inflammatory cell infiltration,islet severe destruction,and islet cells were less;in comparison,human placenta-derived mesenchymal stem cell transplantation group Islet cell response can be seen to be significantly reduced,and the structure is complete,more islet cellsconclusion1.Transplantation of human placenta-derived mesenchymal stem cells into GDM rats through the tail vein can improve blood glucose levels in GDM rats.Pathological results suggest that it may play a role in repairing damaged islet cells. |
PDF Full Text Request