| Objective:Stroke is a disease with high incidence,mortality and disability rate in China,so it poses a great threat to the health of urban and rural residents.Normally,Stroke is divided into ischemic or hemorrhagic stroke,and 80% of stroke patients are caused by cerebral ischemia.The two most common types of ischemic stroke are cholesterol embolic stroke caused by rupture of large atherosclerotic plaques and thromboembolic stroke caused by cardiogenic diseases.Although there were countless studies on the pathophysiology of ischemic stroke,cholesterol embolic stroke and thromboembolic stroke have not been used to be compare in proteomic.The molecular mechanism of cholesterol embolic stroke and thromboembolic stroke is still unclear.The purpose of this study was to explore the difference between cholesterol embolic stroke and thromboembolic stroke in acute phase from the perspectives of animal experiment and proteomic.We hope this study would do benefits to find out the difference between these two kind of stroke and illuminate the potential molecular mechanism,which will provide inspiration and help for the better implementation of accurate treatment in clinical work.Method:Sprague-Dawley rat was chosen to make cerebral microembolic model.TTC staining,Evans Blue staining and HE staining were performed in Thrombus embolization group,Cholesterol embolization group and Sham operation group,and Longa score was used for behavioral assessment.We chose TMT labeled protein spectrum to detect the protein expression profile,and the results were analyzed through GO,KEGG and enrichment analysis.We use SPSS 21.0 as statistical software,and double tail non-paired t test was used to analyze our data,it is considered to be statistically significant that P < 0.05.Results:The infarct volume in cholesterol embolism group was smaller than that in thrombus group.,7.86 ±3.73% VS 20.08 ±9.23% at 6 hours after operation(P< 0.005).The infarct volume was 20.08 ±3.62% VS 25.39 ±4.81% in cholesterol embolism group and thrombus embolism group at 24 hours after operation(P< 0.05).The number of infarction focus in cholesterol embolism group was higher than that in thrombus embolism group,39.67 ±9.58 VS 28.13 ±8.64 at 6h(P< 0.05),36.60 ±5.602 VS 26.50 ±4.76 at 24h(P< 0.005).No significant difference was found in blood-brain barrier injury between the two groups?There were differences in their protein expression.There were 11 up-regulated proteins such as Ubiquitin protein ligase E3 C,and 5 down-regulated proteins including Caveolin-1.GO analysis showed that the differential proteins were mainly concentrated in the positive and negtive regulation of peptidase activity,while in the aspect of cell structure,Golgi complex is a region where differential proteins are mostly concentrated.KEGG pathway analysis suggests that cell adhesion pathway,complement and coagulation pathway,and endocytosis pathway are the most common signaling pathways in which differential proteins are involved.Conclusion:In this study,we found that compared with thromboembolic stroke,cholesterol embolic stroke has a smaller infarct volume,but more number of infarcted focus?There were differences in the protein spectrum in acute stage of embolic stroke.This study may provides a new idea for the molecular mechanism study of stroke caused by shedding atherosclerotic plaque,and may also do help to find more clinical markers of ischemic stroke. |
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