| Pharyngitis is a common respiratory disease,divided into two categories:acute pharyngitis and chronic pharyngitis.Among them,chronic pharyngitis is difficult to cure,easy to ecurrent.chronic pharyngitis is more common than acute pharyngitis and more people suffer from it.The common clinical manifestations include: dry itchy throat,swollen pain,hoarseness,swallowing discomfort or feeling sputum.The pharyngitis brings pain to the patients,which seriously reduces the quality of life of the patients.According to the survey,the prevalence of pharyngitis is increasing year by year.Based on this situation,this topic has developed pharyngitis lozenges.The lozenges is a traditional chinese medicine preparation based on five kinds of chinese herbal medicines such as siraitia grosvenorii,lonicerae japonicae flos,arctii fructus,mume fructus,sterculiae lychnophorae semen.After extracted active ingredients and pressed into tablets,it can alleviate the symptoms of pharyngitis.In this paper,the extraction process,preparation process,quality standard research,stability experiment,preliminary functional experiment and other related research of pharyngitis lozenges are compiled into written materials,which provide reference for related research,as follows:After searching the relevant literature,it was determined that the extraction method of water extraction and alcohol precipitation was used to extract the active ingredients from the herbs.Using the single factor experiment and the response surface analysis method,the extract dry weight and the arctiin content were used as indicators to screen out the best extraction process: extract the medicinal material with9 times of water for 2.5 hours,total extraction 2 times,the filtrate was combined,and then was concentrated to the same volume as the medicinal weight.Ethanol was added to the filtrate to an ethanol concentration of 45%,and allowed to stand for 12 hours,filtered,and the filtrate was evaporated to obtain an extract dry paste.Then preparation process was carried out.According to the nature of the extract,the type of the added auxiliary materials was determined by single factor experiment,and the dosage of the main auxiliary materials was determined by response surface analysis method,and finally the optimal prescription was obtained: extract powder49.96 g,32.53 g of microcrystalline cellulose,6.73 g of sucrose,and 9.29 g of citric acid.The above materials were mixed and then granulated with absolute ethanol.After the granules were dried,1.49 g of menthol powder and 0.2% magnesium stearate were added and then mixing and tableting.Based on the previous research,the quality standard of pharyngitis lozenges was studied.According to the 2015 Pharmacopoeia,a rapid and effective identification of chlorogenic acid and mogroside V was established by thin layer chromatography(TLC).The method was applied to determine the content of arctiin in pharyngitis lozenges by high performance liquid chromatography(HPLC).The obtained linear range was 0.02274 mg/mL to 1.137 mg/mL(r=0.9998),and the average recovery rate was 95.19%(RSD=1.26%),indicating that the method can be used as a method for determining the content of pharyngitis lozenges.Stability studies indicate that the product is stable under the test conditions.Preliminary functional evaluation was performed by rat cotton ball implantation experiment,and the experimental result was positive. |
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