Effects Of Low-level Manganese Exposure On Serum Inflammatory Factors In Workers

Objective To investigate the effects of low-level manganese exposure on blood manganese and serum inflammatory factors in workers,and to explore the relationship between serum manganese and serum inflammatory factors levels and serum inflammatory factors levels,and provide scientific evidence for the prevention and treatment of manganese poisoning.Objective and Methods 108 manganese workers in a manganese operation plant in Guangxi were selected as manganese exposure groups,which were stratified by job type,gender,age and working age;92non-manganese workers were used as control group.Determination of manganese concentration in working environment by inductively coupled plasma mass spectrometry(ICP-MS);determination of manganese concentration in workers by graphite furnace-atomic absorption spectrometry(GFAAS);determination of worker serum by enzyme-linked immunosorbent assay(ELISA)Concentrations of inflammatory factors,including tumor necrosis factor-?(TNF-?),prostaglandin E2(PGE2),interleukin-1?(IL-1?),interleukin 2(IL-2),interleukin-6(IL-6),interleukin-8(IL-8);statistical software SPSS24.0for statistical analysis and analysis.Results(1)The geometric mean concentration of air Mn in the working environment is 0.006mg/m~3,wherein the geometrical average concentration of Mn at the finishing operation point is 0.003mg/m~3,the geometric mean concentration of Mn at the smelting operation point is0.004mg/m~3,and the operating point of the power distribution room is Mn geometry.The average concentration is 0.007 mg/m~3,the geometric mean concentration of Mn at the welding operation point is 0.007 mg/m~3,and the geometric mean concentration of Mn in the non-manganese working environment is 0.001 mg/m~3.(2)The positive rate of the main symptoms of autonomic nerve function in the manganese exposure group was higher than that in the control group(?~2=69.133,P<0.01).In the manganese exposure group,the dioxin headache,memory loss,distraction,and easy fatigue were higher than the control group(P<0.05~0.01).(3)The manganese concentration in the manganese exposure group was higher than that in the control group(P<0.01).(4)Logistic regression analysis showed that gender,age,and working age factors did not affect the blood manganese level of workers,and the manganese in workers exposed to manganese increased(OR=5.32,95%CI:2.7-10.4).(5)The concentrations of serum inflammatory factors TNF-?,PGE2,IL-1?,IL-2,IL-6 and IL-8 in the manganese exposure group were higher than those in the control group(P<0.01).(6)Correlation analysis found that blood manganese concentration was positively correlated with serum TNF-?concentration,serum PGE2concentration,serum IL-1?concentration,serum IL-2 concentration,serum IL-6 concentration,and serum IL-8 concentration(r=0.889,0.816,0.826,0.855,0.875,0.825,P<0.01);serum TNF-?concentration and serum PGE2 concentration,serum IL-1?concentration,serum IL-2concentration,serum IL-6 concentration,serum IL-8 concentration All were positively correlated(r=0.938,0.965,0.982,0.969,0.978,P<0.01);serum PGE2 concentration and serum IL-1?concentration,serum IL-2concentration,serum IL-6 concentration,serum IL-8 concentration were positive Correlation(r=0.982,0.974,0.977,0.967,P<0.01);serum IL-1?concentration was positively correlated with serum IL-2 concentration,serum IL-6 concentration,serum IL-8 concentration(r=0.982,0.974,0.983,P<0.01);serum IL-2 concentration was positively correlated with serum IL-6 concentration and serum IL-8 concentration(r=0.993,0.990,P<0.01);serum IL-6 concentration and serum IL-8 concentration Positive correlation(r=0.982,P<0.01).(7)Multivariate stepwise linear regression analysis showed that blood manganese concentration,TNF-?,IL-6,IL-8 contributed to the regression model(the partial regression coefficients were-2.54,0.223,24.858,0.144,P<0.01).The multiple regression equation with IL-2concentration as the dependent variable was:IL-2 concentration=-118.331-2.054×bloodmanganeseconcentration+0.223×TNF-?concentration+24.858×IL-6 concentration+0.144×IL-8 concentration,R~2=0.995.Conclusions(1)Low levels of manganese exposure can cause elevated blood manganese levels in workers;(2)low levels of manganese exposure can cause an increase in the positive rate of neurological symptoms in workers;(3)low levels of manganese exposure may cause workers to serum TNF-?,PGE2 IL-1?,IL-2,IL-6,IL-8 levels increased;(4)There was a positive correlation between serum manganese and serum inflammatory factors and serum inflammatory factors,suggesting that low levels of manganese exposure may promote increased the effect of serum inflammation in workers.