| Objective:Although radiotherapy has provided the backbone of major treatment and adjuvant therapy for several types of thoracic malignancy,it has long been known that the lungs are sensitive to the deleterious effects of ionizing radiation.Radiation-induced lung injury(RILI)is a common complication after chest radiotherapy,including radiation pneumonia and radiation.induced lung fibrosis(RIL).Radiation-induced lung injury seriously affects the patient's quality of life and subsequent treatment effects,and is an irreversible process,so it is very important to prevent radiation-induced lung injury.Omega-3 fish oil fat emulsion(Juventus)is a commonly used fat emulsion in clinical practice.Currently,it mainly controls body inflammation and immune function,and we know that many experts have studied various uses of omega-3 fish oil fat emulsion,but so far,omega It is not clear whether there is protective effect of-3 fat emulsion on lungs caused by radiation(pneumonia,pulmonary fibrosis).The purpose of this experiment is to explore the effect of omega-3 fish oil fat emulsion on radiation lung injury in mice.Materials and MethodsOne hundred male mice(20-25 g)with no specific pathogen C57BL/6(about 2 months old)were randomly divided into control group(20 animals without any treatment)and high-dose irradiation group(20 Gy 20 single lung irradiation).Only),high-dose irradiation group(omega-3 fish oil fat emulsion treatment and whole lung single irradiation 20Gy 20),simple irradiation group(16Gy single lung irradiation 20),irradiation irradiation group(omega-3)Fish oil fat emulsion treatment and whole lung single irradiation 16Gy 20)Irradiation before irradiation group Mice mice were intraperitoneally injected with omega-3 fish oil fat emulsion(15ml/Kg)daily for 7 days,using 6MV-X line alone In the irradiation group and the irradiation group,20 Gy and 16 Gy were irradiated for a single time(according to the reading of domestic literature).After irradiation,mice in the irradiation group were continuously injected intraperitoneally with omega-3 fish oil fat emulsion(15 ml/Kg)daily for 14 consecutive days.The control group was given on the 2nd,4,8 and 12 weeks after irradiation.Rats,mice in single irradiation group,lung tissue in irradiation group,and right lung tissue 5 ? m sections were completely deparaffinized in xylene,dehydrated by ethanol gradient,stained with hematoxylin-eosin(HE),and Masson stained.The level of pulmonary alveolitis and pulmonary fibrosis and the presence or absence of deposited collagen fibers in irradiated lungs of mice.And immunohistochemistry(Immunohistochemistry IHC)was used to observe the expression of inflammatory factors IL-6 and TGF-?.The left lung tissue was subjected to alkaline hydrolysis to determine the content of hydroxyproline(HYP).In this experiment,SPSS 16.0 software was used for statistical analysis.The results were expressed as x ± s.One-Way Anova analysis was used.P<0.05 was considered statistically significant.Results1.The mice irradiated with 20Gy for a single time died gradually within 2 weeks.The body weight of mice irradiated with either Irradiation or Irradiation decreased significantly,but the mice in the irradiated group were slightly heavier.After 16 Gy Irradiation in Mice:Irradiated mice developed radioactive skin lesions and appeared to have hair loss in about 4 weeks until 12 weeks.The mice in the blank control group had moderate lung tissue size and no adhesion with the surrounding,showing pink,while the mice in the irradiation group and the irradiation group showed adhesions to the thorax at 4 weeks and 8 weeks.At weekdays,mice may have different degree of atrophy of the lung tissue and a smaller size,and the color is dark gray.2.The condition of acute lung injury:Observe the pathological changes of lung tissue in rats after irradiation.In the blank control group,the alveolar structure is normal and there is no mononuclear macrophage infiltration.Irradiation mice:After 2 days of irradiation,inflammatory reaction was observed in the lungs of mice,causing leukocyte infiltration,resulting in alveolar edema and vascular congestion;edema,adhesion,and mononuclear nucleus were observed in the lung tissue at 4 weeks after irradiation.Macrophages,lymphocytes,and other inflammatory exudates,and alveolar septum widened;8 weeks after irradiation,the mouse alveoli began to collapse and fibroblasts proliferated.Obvious fibrosis was observed in the mouse lung tissue at 12 weeks after irradiation.In the irradiation group,the above process was weaker than that of the irradiation alone group.3.Inflammatory factors IL-6 and TGF-p are closely related to radiation-induced lung injury,of which IL-6 is most strongly expressed at 4 and 8 weeks after irradiation,and TGF-? is at 8 and 12 weeks after radiation.The expression was strongest,while the expression of IL-6 and TGF-? in the irradiated group of omega-3 fish oil fat emulsion was weaker than that of the pure irradiation group.4.The relative ratio of the same time period:the content of hydroxyproline in mice of the blank control group<the content of hydroxyproline in the irradiated group<the content of hydroxyproline in the irradiated group(P<0.05).Conclusion20Gy single lung irradiation could not be used to construct a radiation-induced lung injury model in C57BL/6 male mice.The radiation lung injury model can be constructed with 16 Gy single lung irradiation.After pathological analysis and hydroxyproline acid and immunohistochemical analysis,omega-3 fish oil fat emulsion can effectively reduce the radiation lung injury caused by a single irradiation of mice 16Gy,and there is no obvious toxicity to the mouse lung tissue.And ?-3 fish oil fat emulsion may reduce the expression of IL-6 and TGF-?,which weakens the radiation-induced lung injury. |
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