Hub Genes Of Human Bladder Cancer Associated With Prognosis Revealed By WGCNA Method

Background:Bladder cancer was a malignant disease in patients,our research aimed at discovering the possible biomarkers for the diseases.Results:The gene chip GSE31684,including 93samples,was downloaded from the GEO datasets and co-expression network was constructed by the data.Molecular complex detection(MCODE)was used to identify hub genes.The most significant cluster including 16 genes:CDH11,COL3A1,COL6A3,COL5A1,AEBP1,COL1A2,NTM,COL11A1,THBS2,COL8A1,COL1A1,BGN,MMP2,PXDN,THY1,and TGFB1I1 was identified.After annotated by BiNGO,they were suggested associated with collagen fibril organization and blood vessel development.In addition,the Kaplan Meier curves were obtained by UALCAN.The high expression of THY1,AEBP1,CDH11,COL1A1,COL1A2,COL11A1,MMP2,PXDN,BGN,COL5A1,COL8A1,and TGFB1I1 indicated poor prognosis of the patients(P<0.05).Finally,we examined genes’expression between low and high tumor stage by the Wilcoxon test(P<0.05),TGFB1I1 was excluded.Through the analysis of the Connectivity map,we found the drug of purimycin may be the possible drugs for the bladder cancer.The website of SepreSA(http://cpi.bio-x.cn/sepresa/)suggested the drugs may lead heart injury,protein uria,ear harmness,and so on.Conclusion:THY1,AEBP1,CDH11,COL1A1,COL1A2,COL11A1,MMP2,PXDN BGN,COL5A1,COL8A1 associated with the tumor stage as well as tumor patients’ prognosis.COL5A1,COL8A1(P<0.01)may serve as therapeutic targets for the disease.