Study On Anti-influenza Activity Of Zanamivir Conjugates And Screening Of Influenza Antibody

Influenza is one of the infectious diseases with the highest incidence,the fastest spread and the most pathogenic area.Influenza viruses cause epidemics and pandemics in human populations,inflecting enormous suffering and economic losses.Influenza virus has three subtypes(A,B and C).The two glycoproteins,hemagglutinin(HA)and neuraminidase(NA)which are on the surface of the virus envelope are the main targets of drug design.Influenza A virus is the most frequent and harmful of the three subtypes.In this study,the anti-influenza activity research system was established,and the anti-influenza activity of compounds was detected in a stable and rapid way.The NA inhibition results show that the IC50 value of ZA polymer is about 5 nM.Moreover,the cell experiments show that it can inhibite virus in both invasion and release processes.Although the harm of influenza B virus is small,it is one of the components of seasonal influenza virus and can induce a wide range of infection.In this study,we obtained the heavy chain and light chain sequences of antibody by high-throughput sequencing.Then the target genes were synthesized and used for screening antibodies.The neutralization tests reveal that the screened antibody 0809 is a broadly therapeutic antibody that can neutralize influenza B viruses from different lineages.Hemagglutination inhibition experiments show that it is bound to HA of the virus.SPR assays show that the 0809 antibody has strong affinity with antigens.Finally,we cocrystallize antibody complexes in order to know the structural mechanics of antigen and antibody interaction.In conclusion,the anti-influenza activity of multivalent compounds was explored and a broadly neutralizing antibody against influenza B virus was screened by high-throughput sequencing.This has laid the foundation for further research and development of anti-influenza virus drugs.