| [Background]Biomedical polymer materials are a class of macromolecule materials which can repair,substitute and regenerate organism tissues and have special functions.The research field involves materials science,chemistry,medicine and life sciences,etc.With the development of biomedical polymer materials and the increasing demand of human beings for materials,the application of biomedical polymer materials has spread throughout the medical field,such as artificial organs,surgical repair,physiotherapy and rehabilitation,diagnosis and treatment,etc.Polyurethane material is a widely used medical polymer materials with low biotoxicity,excellent mechanical properties and good biocompatibility.However,due the toxicity of isocyanates,the main raw materials for polyurethane and the defects in the manufacturing process,it can cause serious health damage to the professionals.Therefore,the development of healthier and more environment-friendly polyurethane and the biosafety assessment of it have become a hot research topic.In recent years,with the development of fluorescence imaging technology,this technique has become a direct visualization tool for physiological process observation and clinicopathological imaging.Due to the poor photochemical stability,small Stokes shift and large cytotoxicity of traditional organic fluorescent dyes and fluorescent proteins,which can not be ignored,the polymer-based imaging probes have been rapidly developing and applying.[Objective]FPHU is a new type of fluorescent polyurethane material.Its synthesis does not use the toxic raw materials isocyanate,reducing the occupational injury in the process of production.In this study,the biocompatibility of FPHU materials was studied to determine the possibility of its application in the body.At the same time,the application of FPHU as a polymer fluorescent probe was also explored.[Materials and methods]Five kinds of cells was used in the study including the mouse established fibroblast cell(L929),human acute monocytic leukemia cell line THP-1,human pulmonary adenocarcinoma cell line A549,human bronchial epithelial cell line BEAS-2B and the murine macrophage cell line RAW.267.In terms of biocompatibility,with phenol as a positive control group,all of them were treated with FPHU material and its extracts for studying the cytotoxicity.L929 and THP-1 cells were used to exploring the further effect of material extracts on cell viability,apoptosis,cell cycle and inflammatory response.The blood compatibility of FPHU material was evaluated with rabbit blood.For biological applications,we characterized the photophysical properties of FPHU materials and conducted cell imaging experiments on A549,BEAS-2B and L929 cells.At the same time,we compared the biocompatibility and the effect of imaging application of FPHU materials before and after ethanol dialysis purification.[Results]When the concentration of the purified FPHU material was less than 0.3mg/ml,the toxicity of L929,A549,BEAS-2B and RAW.267 was low.After treated with 100%FPHU extract for 48h,the cell viability of L929 cells was still higher than 70%,and the FPHU extract did not cause the apoptosis or cells cycle delay,nor promoted the production of inflammatory factors.The hemolysis rate of FPHU was less than 5%,in line with blood compatibility evaluation criteria of biomedical materials.FPHU emited stable fluorescence at a wavelength of 360 nm,with a quantum yield of 23.6%and excellent photobleaching resistance.Imaging experiments had shown that it could be imaged on L929,A549 and BEAS-2B with stable fluorescence.[Conclusion]The purified FPHU material had good biocompatibility and excellent fluorescence properties,which could be used as biomedical materials.However,in terms of imaging application,it was less efficient to get into cells,resulting in poor cell imaging.Thus,further improvements are needed for cell imaging application.The purification method of ethanol dialysis could obviously improve the biocompatibility and the cell imaging effect of FPHU materials. |
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